The association between HERG gene expression and cardiac arrhythmia disease in children

Fu,Mingyu; Li,Chunli; Wang,Qingwen; Wang,Yan; An,Xinjiang

doi:10.3892/etm.2016.3802

The association between HERG gene expression and cardiac arrhythmia disease in children

Authors:
- Mingyu Fu
- Chunli Li
- Qingwen Wang
- Yan Wang
- Xinjiang An
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Affiliations: Department of Cardiology, Xuzhou Children's Hospital, Xuzhou, Jiangsu 221002, P.R. China
Published online on: October 12, 2016 https://doi.org/10.3892/etm.2016.3802
Pages: 3567-3570
Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

We explored the possible link between the expression of HERG gene and cardiomyopathy in children. From April 2013 to April 2015, 73 children with cardiac arrhythmia who were treated were enrolled in the present study to serve as the observation group. At the same time, 76 normal individuals were also enrolled as the control group. HERG expression level in the observation group was compared with the control group. To determine the level of HERG gene expression we used fluorescent directional PCR, enzyme immunoassay and western blot analysis. The results showed that HERG mRNA level in the observation group was significantly higher than that of the control group. The level of HERG protein in the observation group was significantly higher as well. In the observation group, HERG expression gradually increased with time during the course of the disease. This result suggested that HERG gene expression was associated with the severity of cardiac arrhythmia in children. HERG expression may be the cause of deterioration in cardiomyopathy. The results have provided a theoretical and practical basis for the diagnosis and treatment of children cardiomyopathy. Thus, we established a correlation between HERG expression and cardiac arrhythmia in children.

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