Open Access

Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin

  • Authors:
    • Liang Zhao
    • Yu Jing
    • Lin Qu
    • Xiangwei Meng
    • Yang Cao
    • Huibing Tan
  • View Affiliations

  • Published online on: October 21, 2016     https://doi.org/10.3892/etm.2016.3832
  • Pages: 3680-3684
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The expression of adrenomedullin (ADM) in injured tissue of rat spinal cord was observed and the effect of recombinant human erythropoietin was analyzed. A total of 45 Sprague‑Dawley rats were selected and divided into 3 equal groups including, a sham-operation group in which rats received an excision of vertebral plate; a spinal cord injury model group and a recombinant human erythropoietin group in which rats with spinal cord injury received a caudal vein injection of 300 units recombinant human erythropoietin after injury. Hematoxylin and eosin staining was performed to observe the spinal cord injury conditions. Immunohistochemical staining was performed to observe the expression of ADM. Pathologic changes in the group of recombinant human erythropoietin at various times were significantly less severe than those in the group of spinal cord injury model. The expression of ADM was increased particularly in the group of recombinant human erythropoietin (P<0.01). The improved Tarlov scores of the group of spinal cord injury model and the group of recombinant human erythropoietin were lower than those of the sham‑operation group at 3, 6 and 9 days (P<0.01). Thus, the recombinant human erythropoietin is capable of alleviating the secondary injury of spinal cord. One of the mechanisms may be achieved by promoting the increase of ADM expression.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhao L, Jing Y, Qu L, Meng X, Cao Y and Tan H: Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin. Exp Ther Med 12: 3680-3684, 2016
APA
Zhao, L., Jing, Y., Qu, L., Meng, X., Cao, Y., & Tan, H. (2016). Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin. Experimental and Therapeutic Medicine, 12, 3680-3684. https://doi.org/10.3892/etm.2016.3832
MLA
Zhao, L., Jing, Y., Qu, L., Meng, X., Cao, Y., Tan, H."Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin". Experimental and Therapeutic Medicine 12.6 (2016): 3680-3684.
Chicago
Zhao, L., Jing, Y., Qu, L., Meng, X., Cao, Y., Tan, H."Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3680-3684. https://doi.org/10.3892/etm.2016.3832