Open Access

Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia

  • Authors:
    • Jianping Wei
    • Qingmei Nie
    • Feng Li
  • View Affiliations

  • Published online on: October 14, 2016     https://doi.org/10.3892/etm.2016.3806
  • Pages: 3694-3698
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the effect of lamotrigine and the expression of myeloid-related protein 8 (MRP8) and interleukin-7 (IL-7) in the treatment of focal cortical dysplasia with secondary intractable epilepsy. In this study, rats with focal cortical dysplasia with secondary intractable epilepsy (constructed by our laboratory) were selected and used for experimentation, 21-day Sprague‑Dawley rats were randomly divided into the control group (38 rats), the observation group Ⅰ (39 rats), and the observation group Ⅱ (38 rats). Rats in the observation group Ⅰ received daily intraperitoneal injection of 0.02 mg/kg lamotrigine, and those in the observation group Ⅱ and the control group received daily intraperitoneal injection of 0.02 mg/kg normal saline. Expression quantities of MRP8 and IL-7 in the hippocampus sample tissues of mice in the control group, observation group Ⅰ, and observation group Ⅱ were measured via fluorescence quantitative polymerase chain reaction assay, western blot analysis, enzyme-linked immunosorbent assay, and immunohistochemistry 48 h later. MRP8 and IL-7 gene mRNA levels of the control group, the observation group Ⅰ and the observation group Ⅱ had no significant differences (P>0.05). The expression quantity on the protein level of MRP8 and IL-7 showed no significant differences (P>0.05) between the observation group Ⅰ (7.91±1.3, 3.86±0.38) and the control group (7.52±1.03, 3.62±0.29). The expression quantity of MRP8 and IL-7 showed significant differences (P<0.05) between observation group Ⅱ (27.47±1.13, 19.45±0.48) and observation group Ⅰ (7.91±1.3, 3.86±0.38). It was found that MRP8 and IL-7 were focused on the nerve cell membrane of hippocampus of rats in the observation group by immunohistochemistry experiments. In conclusion, the results from the present study show that lamotrigine can be used to treat rats with focal cortical dysplasia with secondary intractable epilepsy by reducing the expression levels of MRP8 and IL-7 in the body, providing a new therapeutic target to the follow-up treatment of focal cortical dysplasia with secondary intractable disease.
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December-2016
Volume 12 Issue 6

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Spandidos Publications style
Wei J, Nie Q and Li F: Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia. Exp Ther Med 12: 3694-3698, 2016
APA
Wei, J., Nie, Q., & Li, F. (2016). Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia. Experimental and Therapeutic Medicine, 12, 3694-3698. https://doi.org/10.3892/etm.2016.3806
MLA
Wei, J., Nie, Q., Li, F."Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia". Experimental and Therapeutic Medicine 12.6 (2016): 3694-3698.
Chicago
Wei, J., Nie, Q., Li, F."Lamotrigine decreases MRP8 and IL-7 in rat models of intractable epilepsy secondary to focal cortical dysplasia". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3694-3698. https://doi.org/10.3892/etm.2016.3806