Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy

Zhao,Huazhou; Xi,Hongqing; Wei,Bo; Cai,Aizhen; Wang,Ting; Wang,Yi; Zhao,Xudong; Song,Yanjing; Chen,Lin

doi:10.3892/etm.2016.3908

Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy

Authors:
- Huazhou Zhao
- Hongqing Xi
- Bo Wei
- Aizhen Cai
- Ting Wang
- Yi Wang
- Xudong Zhao
- Yanjing Song
- Lin Chen
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Affiliations: Department of General Surgery, General Hospital of Chinese People's Liberation Army, Beijing 100853, P.R. China, Medical Administration Division of Beijing Chaoyang Hospital, Beijing 100020, P.R. China
Published online on: November 15, 2016 https://doi.org/10.3892/etm.2016.3908
Pages: 3885-3892
Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of this study was to investigate the expression of decorin (DCN) in the intestinal tissues of mice with inflammatory bowel disease (IBD) and its correlation with autophagy. The IBD mouse model was created by intrarectal injection of trinitrobenzene sulfonic acid. The pathology of colon tissues of the mice was examined using hematoxylin and eosin staining. Expression of DCN and the proteins associated with autophagy was detected using immunohistochemistry. Normal human colon mucosal epithelial cells (NCM460 cells) were transfected with DCN expression plasmid and the expression of DCN and autophagy‑associated proteins was detected by western blot analysis. Cell apoptosis was studied using an Annexin V apoptosis detection assay and intracellular autophagosomes were observed using electron microscopy. The IBD mouse model was successfully established. Thickening, edema and inflammatory cell infiltration of the intestinal wall were observed in the IBD mice. The expression of DCN as well as the autophagy‑associated proteins beclin 1 and LC3B, was increased in the intestinal tissues of the IBD mice. Furthermore, in the NCM460 cells transfected with DCN, the expression of beclin 1 and LC3B was upregulated, while p62 expression was downregulated. Intracellular autophagosomes were increased and apoptosis was decreased in the cells with DCN overexpression. Inhibition of autophagy reversed the effects of DCN on apoptosis. Therefore, DCN is able to induce autophagy and protect intestinal cells during the occurrence and development of IBD.

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1	Agilli M, Aydin FN, Cayci T and Kurt YG: Assessment of active mucosal inflammation in IBD patients in clinical remission. J Gastrointestin Liver Dis. 23:462–463. 2014.PubMed/NCBI
2	Dunkin D, Mehandru S and Colombel JF: Immune cell therapy in IBD. Dig Dis. 32:(Suppl 1). S61–S66. 2014. View Article : Google Scholar
3	Hildner K, Punkenburg E, Abendroth B and Neurath MF: Immunopathogenesis of IBD: Batf as a Key Driver of Disease Activity. Dig Dis 34 (Suppl 1). 40–47. 2016.
4	De Salvo C, Ray S and Pizarro TT: Mechanisms and models for intestinal fibrosis in IBD. Dig Dis. 32:(Suppl 1). S26–S34. 2014. View Article : Google Scholar
5	Latella G, Di Gregorio J, Flati V, Rieder F and Lawrance IC: Mechanisms of initiation and progression of intestinal fibrosis in IBD. Scand J Gastroenterol. 50:53–65. 2015. View Article : Google Scholar : PubMed/NCBI
6	Bryant RV, Brain O and Travis SP: Conventional drug therapy for inflammatory bowel disease. Scand J Gastroenterol. 50:90–112. 2015. View Article : Google Scholar : PubMed/NCBI
7	Chen M, Yi F, Zhou F, Huang M, Li J, Yan W, Li L and Xia B: Risk factors for initial surgery in patients with Crohn's disease in Central China. Surg Today. 45:1002–1008. 2015. View Article : Google Scholar : PubMed/NCBI
8	Connelly TM, Koltun WA, Sangster W, Berg AS, Hegarty JP, Harris L III, Deiling S and Stewart DB: An interleukin-4 polymorphism is associated with susceptibility to Clostridium difficile infection in patients with inflammatory bowel disease: Results of a retrospective cohort study. Surgery. 156:769–774. 2014. View Article : Google Scholar : PubMed/NCBI
9	Katz LH, Kopylov U, Fudim E, Yavzori M, Picard O, Ungar B, Eliakim R, Ben-Horin S and Chowers Y: Expression of IL-2, IL-17 and TNF-alpha in patients with Crohn's disease treated with anti-TNF antibodies. Clin Res Hepatol Gastroenterol. 38:491–498. 2014. View Article : Google Scholar : PubMed/NCBI
10	Zatorski H, Marynowski M and Fichna J: Is insulin-like growth factor 1 (IGF-1) system an attractive target inflammatory bowel diseases? Benefits and limitation of potential therapy. Pharmacol Rep. 68:809–815. 2016. View Article : Google Scholar : PubMed/NCBI
11	Nata T, Fujiya M, Ueno N, Moriichi K, Konishi H, Tanabe H, Ohtake T, Ikuta K and Kohgo Y: MicroRNA-146b improves intestinal injury in mouse colitis by activating nuclear factor-kB and improving epithelial barrier function. J Gene Med. 15:249–260. 2013. View Article : Google Scholar : PubMed/NCBI
12	Chen JY, Hour TC, Yang SF, Chien CY, Chen HR, Tsai KL, Ko JY and Wang LF: Autophagy is deficient in nasal polyps: Implications for the pathogenesis of the disease. Int Forum Allergy Rhinol. 5:119–123. 2015. View Article : Google Scholar : PubMed/NCBI
13	Liu H, He Z and Simon HU: Protective role of autophagy and autophagy-related protein 5 in early tumorigenesis. J Mol Med (Berl). 93:159–164. 2015. View Article : Google Scholar : PubMed/NCBI
14	Annese V, Duricova D, Gower-Rousseau C, Jess T and Langholz E: Impact of New Treatments on Hospitalisation, Surgery, Infection, and Mortality in IBD: A Focus Paper by the Epidemiology Committee of ECCO. J Crohns Colitis. 10:216–225. 2016. View Article : Google Scholar : PubMed/NCBI
15	Murthi P, van Zanten DE, Eijsink JJ, Borg AJ, Stevenson JL, Kalionis B, Chui AK, Said JM, Brennecke SP and Erwich JJ: Decorin expression is decreased in first trimester placental tissue from pregnancies with small for gestation age infants at birth. Placenta. 45:58–62. 2016. View Article : Google Scholar : PubMed/NCBI
16	Nemani N, Santo L, Eda H, Cirstea D, Mishima Y, Patel C, O'Donnell E, Yee A and Raje N: Role of decorin in multiple myeloma (MM) bone marrow microenvironment. J Bone Miner Res. 30:465–470. 2015. View Article : Google Scholar : PubMed/NCBI
17	Esmaeili M, Berry M, Logan A and Ahmed Z: Decorin treatment of spinal cord injury. Neural Regen Res. 9:1653–1656. 2014. View Article : Google Scholar : PubMed/NCBI
18	Kasamatsu A, Uzawa K, Minakawa Y, Ishige S, Kasama H, Endo-Sakamoto Y, Ogawara K, Shiiba M, Takiguchi Y and Tanzawa H: Decorin in human oral cancer: A promising predictive biomarker of S-1 neoadjuvant chemosensitivity. Biochem Biophys Res Commun. 457:71–76. 2015. View Article : Google Scholar : PubMed/NCBI
19	Araki K, Wakabayashi H, Shintani K, Morikawa J, Matsumine A, Kusuzaki K, Sudo A and Uchida A: Decorin suppresses bone metastasis in a breast cancer cell line. Oncology. 77:92–99. 2009. View Article : Google Scholar : PubMed/NCBI
20	Reed CC, Waterhouse A, Kirby S, Kay P, Owens RT, McQuillan DJ and Iozzo RV: Decorin prevents metastatic spreading of breast cancer. Oncogene. 24:1104–1110. 2005. View Article : Google Scholar : PubMed/NCBI
21	Obba S, Hizir Z, Boyer L, Selimoglu-Buet D, Pfeifer A, Michel G, Hamouda MA, Gonçalvès D, Cerezo M, Marchetti S, et al: The PRKAA1/AMPKα1 pathway triggers autophagy during CSF1-induced human monocyte differentiation and is a potential target in CMML. Autophagy. 11:1114–1129. 2015. View Article : Google Scholar : PubMed/NCBI
22	Cheng X, Zhang X, Su J, Zhang Y, Zhou W, Zhou J, Wang C, Liang H, Chen X, Shi R, et al: miR-19b downregulates intestinal SOCS3 to reduce intestinal inflammation in Crohn's disease. Sci Rep. 5:103972015. View Article : Google Scholar : PubMed/NCBI
23	Zeng TS, Liu FM, Zhou J, Pan SX, Xia WF and Chen LL: Depletion of Kupffer cells attenuates systemic insulin resistance, inflammation and improves liver autophagy in high-fat diet fed mice. Endocr J. 62:615–626. 2015. View Article : Google Scholar : PubMed/NCBI
24	Mauviel A, Santra M, Chen YQ, Uitto J and Iozzo RV: Transcriptional regulation of decorin gene expression. Induction by quiescence and repression by tumor necrosis factor-alpha. J Biol Chem. 270:11692–11700. 1995. View Article : Google Scholar : PubMed/NCBI
25	Schaefer L, Macakova K, Raslik I, Micegova M, Gröne HJ, Schönherr E, Robenek H, Echtermeyer FG, Grässel S, Bruckner P, et al: Absence of decorin adversely influences tubulointerstitial fibrosis of the obstructed kidney by enhanced apoptosis and increased inflammatory reaction. Am J Pathol. 160:1181–1191. 2002. View Article : Google Scholar : PubMed/NCBI
26	Tomasello G, Sinagra E, Raimondo D, Palumbo VD, Puleio R, Cottone M, Damiani P, Traina G, Abruzzo A, Damiani F, et al: Validation of a modified model of TNBS-induced colitis in rats. How to induce a chemical colitis in rats. Acta Biomed. 86:92–96. 2015.PubMed/NCBI