Open Access

Biological characterization of sheep kidney‑derived mesenchymal stem cells

  • Authors:
    • Meng Ji
    • Chunyu Bai
    • Lu Li
    • Ya'nan Fan
    • Caiyun Ma
    • Xiangchen Li
    • Weijun Guan
  • View Affiliations

  • Published online on: November 15, 2016     https://doi.org/10.3892/etm.2016.3902
  • Pages: 3963-3971
  • Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to isolate, culture and characterize sheep metanephric mesenchymal stem cells (MMSCs). The MMSCs were isolated from the kidney tissue of six‑week‑old sheep fetus. This study investigated whether primary MMSCs could be grown for 26 passages and expressed Oct‑4, which is involved in the self‑renewal of undifferentiated pluripotent stem cells. The MMSCs also expressed the renal lineage marker gene PAX2, and mesenchymal cell marker genes CD44, FN1 and VIM. Expression of these genes was detected using immunofluorescence and reverse transcription‑polymerase chain reaction assays. Additionally, we observed that the MMSCs are able to differentiate into adipocyte, hepatocyte and chondrocyte cells. Karyotype analyses showed that these cells were 95% diploid and thus differentiated. These results indicate that the MMSCs obtained from sheep fetuses possessed certain characteristics of multipotent stem cells. Therefore, MMSCs may potentially offer utility for tissue engineering and cellular transplantation therapy, and further studies are required to investigate these uses.
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December-2016
Volume 12 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ji M, Bai C, Li L, Fan Y, Ma C, Li X and Guan W: Biological characterization of sheep kidney‑derived mesenchymal stem cells. Exp Ther Med 12: 3963-3971, 2016
APA
Ji, M., Bai, C., Li, L., Fan, Y., Ma, C., Li, X., & Guan, W. (2016). Biological characterization of sheep kidney‑derived mesenchymal stem cells. Experimental and Therapeutic Medicine, 12, 3963-3971. https://doi.org/10.3892/etm.2016.3902
MLA
Ji, M., Bai, C., Li, L., Fan, Y., Ma, C., Li, X., Guan, W."Biological characterization of sheep kidney‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12.6 (2016): 3963-3971.
Chicago
Ji, M., Bai, C., Li, L., Fan, Y., Ma, C., Li, X., Guan, W."Biological characterization of sheep kidney‑derived mesenchymal stem cells". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3963-3971. https://doi.org/10.3892/etm.2016.3902