MicroRNA‑212 suppresses the proliferation and migration of osteosarcoma cells by targeting forkhead box protein A1 Retraction in /10.3892/etm.2021.10409

Liu,Jian; Chen,Bohua; Yue,Bin; Yang,Junde

doi:10.3892/etm.2016.3880

MicroRNA‑212 suppresses the proliferation and migration of osteosarcoma cells by targeting forkhead box protein A1

Retraction in: /10.3892/etm.2021.10409

Authors:
- Jian Liu
- Bohua Chen
- Bin Yue
- Junde Yang
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Affiliations: Department of Orthopedics, Eighth People's Hospital of Qingdao, Medical School of Qingdao University, Qingdao, Shandong 266100, P.R. China
Published online on: November 7, 2016 https://doi.org/10.3892/etm.2016.3880
Pages: 4135-4141

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Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that function as critical gene regulators by targeting the 3' untranslated region (UTR) of mRNA, causing translational repression or mRNA degradation. Deregulation of specific miRNAs, including miR‑212, has been identified in patients with osteosarcoma. However, the underlying mechanism is yet to be fully elucidated. The present study aimed to reveal the regulatory mechanism of miR‑212 in osteosarcoma cell viability and migration. Quantitative polymerase chain reaction data revealed that miR‑212 was significantly downregulated in osteosarcoma tissues compared with normal bone tissues. miR‑212 was also downregulated in osteosarcoma cell lines compared with normal osteoblast cell lines. Overexpression of miR‑212 significantly suppressed the viability and migration of human osteosarcoma MG‑63 and Saos‑2 cell lines. In addition, forkhead box protein A1 (FOXA1), an oncogene in osteosarcoma, was predicted to be a putative target of miR‑212 by bioinformatical analysis. Furthermore, luciferase reporter assay data confirmed that miR‑212 could directly bind to the seed sequences within the 3'UTR of FOXA1 mRNA, and miR‑212 negatively mediated the protein levels of FOXA1 in osteosarcoma MG‑63 and Saos‑2 cells. Moreover, knockdown of FOXA1 also led to a significant decrease in the viability and migration of osteosarcoma MG‑63 and Saos‑2 cells and the expression levels of FOXA1 were significantly upregulated in osteosarcoma tissues and cell lines. These data suggest that miR‑212 inhibits the viability and migration of osteosarcoma cells by targeting FOXA1. Accordingly, miR‑212 may become a potential candidate for osteosarcoma therapy.

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