TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque

Su,Yijin; Xu,Changsong; Li,Kunyu; Wang,Bo; Chen,Jufang; Liu,Long; Lin,Lizhou; Dong,Qingqing; Du,Lianfang

doi:10.3892/etm.2017.4101

TGF-β1 and TIMP1 double directional rAAV targeted by UTMD in atherosclerotic vulnerable plaque

Authors:
- Yijin Su
- Changsong Xu
- Kunyu Li
- Bo Wang
- Jufang Chen
- Long Liu
- Lizhou Lin
- Qingqing Dong
- Lianfang Du
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Affiliations: Department of Ultrasound, Shanghai General Hospital Affiliated to Nanjing Medical University, Shanghai 200080, P.R. China
Published online on: February 6, 2017 https://doi.org/10.3892/etm.2017.4101
Pages: 1465-1469
Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, we determined whether ultrasound-targeted microbubble destruction (UTMD) combined with dual targeting of transforming growth factor (TGF)-β1 and tissue inhibitors of metalloproteinase (TIMP) 1 recombinant adeno-associated virus (rAAV) can stabilize atherosclerotic vulnerable plaques. First, we used rabbit model to detect the TGF-β1/TIMP1 virus therapy result. H&E staining was used to evaluate the tissues. The protein levels of TGF-β1 and TIMP1 were detected in rabbit models. The THP-1 cells were induced into macrophages, and transfected with TGF-β1 and TIMP1 rAAV under optimized UTMD. The expression of TGF-β1 and TIMP1 was measured by RT-PCR and western blotting. We found that the apoptotic rates were induced when compared to the control group. The rAAV virus group showed a significant decrease in the p-ERT and p-AKT expression. These data support the hypothesis that TGF-β1 and TIMP1 are crucial in the regulation of atherosclerotic plaques.

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