Open Access

Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats

  • Authors:
    • Jinyan Zhao
    • Haixia Hu
    • Yun Wan
    • Yuchen Zhang
    • Liangpu Zheng
    • Zhenfeng Hong
  • View Affiliations

  • Published online on: March 2, 2017     https://doi.org/10.3892/etm.2017.4174
  • Pages: 1820-1826
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Liver damage results from a variety of insults, including hepatitis and chemical toxicity from alcohol, drugs and other toxins. The present study evaluated the hepatoprotective effects and potential mechanisms of action of the Traditional Chinese Medicine Pien Tze Huang Gan Bao (GB) in a rat model of carbon tetrachloride (CCl4)‑induced liver injury. Sixty male Sprague‑Dawley rats were randomly divided into six different groups: i) Control, ii) CCl4 injury model and groups treated with iii) silymarin as a positive drug control, iv) 150 mg/kg GB, v) 300 mg/kg GB and vi) 600 mg/kg GB. Control rats received no treatment, while the remaining ones were intraperitoneally injected with CCl4 (2 ml/kg) to induce acute liver disease. Silymarin or GB was orally administered prior to CCl4 treatment in various treatment groups for 7 days. Animals were sacrificed 24 h post‑CCl4 injection. It was revealed that GB significantly reduced serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transpeptidase and total bilirubin levels in the serum induced by CCl4. BG also prevented CCl4‑induced changes in liver tissues, as revealed by histopathological analysis. CCl4‑induced reductions in endogenous liver antioxidant enzyme activities of superoxide dismutase, glutathione and glutathione peroxidase as well as increases in malondialdehyde and thiobarbituric acid reactive substances were inhibited by GB treatment. Activated NF‑κB in liver tissues was also significantly increased by CCl4, which was attenuated by GB as indicated by immunohistochemical and PCR analysis. Furthermore, CCl4‑mediated increases in the inflammatory factors tumor necrosis factor‑alpha and interleukin‑1β secretion into the serum and their expression in liver tissues were reversed following GB treatment, as revealed by ELISA and PCR, respectively. These findings suggested that GB protects against CCl4‑induced hepatic injury, inflammation and oxidative damage in rats and may be useful in future clinical application of liver injury and disease.
View Figures
View References

Related Articles

Journal Cover

May-2017
Volume 13 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao J, Hu H, Wan Y, Zhang Y, Zheng L and Hong Z: Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats. Exp Ther Med 13: 1820-1826, 2017.
APA
Zhao, J., Hu, H., Wan, Y., Zhang, Y., Zheng, L., & Hong, Z. (2017). Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats. Experimental and Therapeutic Medicine, 13, 1820-1826. https://doi.org/10.3892/etm.2017.4174
MLA
Zhao, J., Hu, H., Wan, Y., Zhang, Y., Zheng, L., Hong, Z."Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats". Experimental and Therapeutic Medicine 13.5 (2017): 1820-1826.
Chicago
Zhao, J., Hu, H., Wan, Y., Zhang, Y., Zheng, L., Hong, Z."Pien Tze Huang Gan Bao ameliorates carbon tetrachloride-induced hepatic injury, oxidative stress and inflammation in rats". Experimental and Therapeutic Medicine 13, no. 5 (2017): 1820-1826. https://doi.org/10.3892/etm.2017.4174