Open Access

Beneficial effects of Jiawei Shenqi-wan and treadmill training on deficits associated with neonatal hypoxic-ischemia in rats

  • Authors:
    • Ha Neui Kim
    • Malk Eun Pak
    • Myung Jun Shin
    • Soo Yeon Kim
    • Yong Beom Shin
    • Young Ju Yun
    • Hwa Kyoung Shin
    • Byung Tae Choi
  • View Affiliations

  • Published online on: March 30, 2017     https://doi.org/10.3892/etm.2017.4286
  • Pages: 2134-2142
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Jiawei Shenqi-wan (JSQW), which comprises Shenqi-wan and two additional medicinal herbs, has been widely used for the treatment of various growth impairments, including cerebral palsy. In the present study, JSQW was administered to hypoxic-ischemic Sprague-Dawley rats that underwent treadmill training from 4‑7 weeks of age to examine the beneficial effects of combined JSQW and treadmill therapy. Behavioral examinations were performed and a significant improvement in cylinder test performance was observed in rats treated with treadmill training compared with hypoxic‑ischemia rats (P<0.05), as well as a significant improvement in passive avoidance test performance for rats treated with JSQW (P<0.05). The thickness of the corpus callosum and the integrated optical density (IOD) of myelin basic protein (MBP) were significantly increased by treatment with treadmill therapy alone (P<0.01 and P<0.001, respectively) and treatment with both JSQW and treadmill significantly increased the IOD of MBP compared with hypoxic‑ischemia rats (P<0.001). Western blot analysis revealed that the expression of neuronal nuclei (NeuN) and doublecortin (Dcx) significantly decreased (P<0.001 and P<0.05, respectively) and MBP expression markedly decreased in the ipsilateral subventricular zone of hypoxic‑ischemic rats compared with the control group; however, the expression of NeuN was significantly recovered by treatment with both JSQW and treadmill training (P<0.05). Furthermore, Dcx expression was significantly recovered by treatment with JSQW (P<0.05), and MBP expression was significantly restored by treatment with treadmill training (P<0.01). In the immunohistochemical analyses, a significant increase in the number of bromodeoxyuridine (BrdU) positive cells in this region was observed in treadmill‑treated rats (P<0.05), whereas significant increases in the number of Brdu/Dcx or NeuN or glial fibrillary acidic protein double‑positive cells were observed only in the group co‑treated with JSQW and treadmill (P<0.01, P<0.05 and P<0.001, respectively). These results suggest that JSQW and treadmill training may contribute to behavior recovery following hypoxic‑ischemia, and JSQW treatment was particularly effective in promoting memory function via enhancing the differentiation of neuronal progenitor cells. The results of the present study therefore suggest that JSQW may provide an additional treatment option for functional recovery with treadmill training in cerebral palsy.
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May-2017
Volume 13 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kim HN, Pak ME, Shin MJ, Kim SY, Shin YB, Yun YJ, Shin HK and Choi BT: Beneficial effects of Jiawei Shenqi-wan and treadmill training on deficits associated with neonatal hypoxic-ischemia in rats. Exp Ther Med 13: 2134-2142, 2017
APA
Kim, H.N., Pak, M.E., Shin, M.J., Kim, S.Y., Shin, Y.B., Yun, Y.J. ... Choi, B.T. (2017). Beneficial effects of Jiawei Shenqi-wan and treadmill training on deficits associated with neonatal hypoxic-ischemia in rats. Experimental and Therapeutic Medicine, 13, 2134-2142. https://doi.org/10.3892/etm.2017.4286
MLA
Kim, H. N., Pak, M. E., Shin, M. J., Kim, S. Y., Shin, Y. B., Yun, Y. J., Shin, H. K., Choi, B. T."Beneficial effects of Jiawei Shenqi-wan and treadmill training on deficits associated with neonatal hypoxic-ischemia in rats". Experimental and Therapeutic Medicine 13.5 (2017): 2134-2142.
Chicago
Kim, H. N., Pak, M. E., Shin, M. J., Kim, S. Y., Shin, Y. B., Yun, Y. J., Shin, H. K., Choi, B. T."Beneficial effects of Jiawei Shenqi-wan and treadmill training on deficits associated with neonatal hypoxic-ischemia in rats". Experimental and Therapeutic Medicine 13, no. 5 (2017): 2134-2142. https://doi.org/10.3892/etm.2017.4286