Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells

  • Authors:
    • Ping Zhang
    • Chao Wu
    • Xiao‑Hui Huang
    • Chen‑Lin Shen
    • Lin Li
    • Wei Zhang
    • Cheng‑Zeng Yao
  • View Affiliations

  • Published online on: October 3, 2017     https://doi.org/10.3892/etm.2017.5252
  • Pages:5597-5604
Metrics: PDF 0 views | HTML 0 views
0

Abstract

Numerous studies have indicated that the expression of matrix metalloproteinase‑9 (MMP‑9) contributes to the atherosclerotic plaque hemorrhage and rupture. Aspirin, a non‑steroidal anti‑inflammation drug, has been known for its anti‑platelet effect in the prevention of the vascular complications of atherosclerosis. The present study aimed to investigate the pharmacological effects of aspirin on tumor necrosis factor‑α (TNF‑α)‑induced MMP‑9 expression and the underlying molecular mechanisms in murine macrophage RAW264.7 cells. Western blot analysis indicated that the protein level of MMP‑9 was reduced by aspirin in a dose‑dependent manner. In addition, downregulation of MMP‑9 mRNA and activity were detected in aspirin‑treated cells using quantitative polymerase chain reaction and a gelatin zymography assay separately. It was also observed that aspirin has a suppressive effect on the activation of nuclear factor (NF)‑κB and inhibits the phosphorylation of mitogen‑activated protein kinases (MAPKs), including extracellular signal‑regulated kinases 1/2, p38 and c‑Jun N‑terminal kinase. Furthermore, subsequent to inhibition of the MAPK pathway by specific inhibitors (PD98059, SB203580 and SP600125), the expression of MMP‑9 was reduced, indicating that the inhibitory effect of aspirin on MMP‑9 in TNF‑α‑treated RAW264.7 cells may be, at least in part, through suppression of NF‑κB activation and the MAPK pathway. These findings support the notion that aspirin has therapeutic potential application in the prevention and treatment of atherosclerosis disease.

Related Articles

Journal Cover

December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhang, P., Wu, C., Huang, X., Shen, C., Li, L., Zhang, W., & Yao, C. (2017). Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells. Experimental and Therapeutic Medicine, 14, 5597-5604. https://doi.org/10.3892/etm.2017.5252
MLA
Zhang, P., Wu, C., Huang, X., Shen, C., Li, L., Zhang, W., Yao, C."Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells". Experimental and Therapeutic Medicine 14.6 (2017): 5597-5604.
Chicago
Zhang, P., Wu, C., Huang, X., Shen, C., Li, L., Zhang, W., Yao, C."Aspirin suppresses TNF‑α‑induced MMP‑9 expression via NF‑κB and MAPK signaling pathways in RAW264.7 cells". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5597-5604. https://doi.org/10.3892/etm.2017.5252