Open Access

Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis

  • Authors:
    • Zhen‑Zhen Chen
    • Xin Gong
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5280
  • Pages:5743-5750
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aims of the present study were to investigate the effects of a traditional Chinese medicine, Hua Yu Xiao Zheng (HYXZ) decoction, on surgically induced endometriosis in a rat model and to determine the possible underlying regulatory mechanisms. A total of 108 female Sprague‑Dawley rats were divided into the control group (n=12) and endometriosis group (EM group; n=96), in which endometriosis was surgically induced in model rats by autotransplantation of endometrial tissues and 72 rats survived. After 3 weeks, the EM model rats were randomly divided into four subgroups (n=18), including the untreated model group, and three groups administered 7, 14 or 21 g/kg HYXZ decoction. Following 28 days of treatment, the associated proteins and genes of ectopic endometrial tissues were analyzed using immunohistochemistry, western blotting and quantitative polymerase chain reaction to investigate the underlying mechanisms. Compared with the model group, the size of the endometriotic implants decreased significantly in the HYXZ‑treated groups. Furthermore, the expression levels of vascular endothelial growth factor (VEGF) and angiopoietin‑2 (Ang‑2) were significantly decreased in HYXZ‑treated groups compared with the model group. These results indicate that HYXZ affected the inhibition of angiogenesis and decreased the endometriotic implant volumes and histopathological scores. The effectiveness of HYXZ may be partially attributed to the decrease of VEGF and Ang‑2 expression levels in the ectopic endometrium.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Chen, Z., & Chen, Z. (2017). Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis. Experimental and Therapeutic Medicine, 14, 5743-5750. https://doi.org/10.3892/etm.2017.5280
MLA
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14.6 (2017): 5743-5750.
Chicago
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5743-5750. https://doi.org/10.3892/etm.2017.5280