Open Access

Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice

  • Authors:
    • Ya‑Jun Lin
    • Yong‑Zhan Zhen
    • Jing‑Bo Wei
    • Jie Wei
    • Jing Dai
    • Jun‑Ling Gao
    • Kai‑Ji Li
    • Gang Hu
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5283
  • Pages:5801-5808
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to assess the protective effects of rhein lysinate (RHL) in a KK/HlJ mouse model of diabetic nephropathy (DN) and to explore its mechanism of action. A total of 4 groups were established: C57BL/J control, the KK/HlJ model and 25 and 50 mg/kg/day RHL‑treated KK/HlJ groups. The KK/HlJ mouse model of DN was established by streptozotocin injection, followed by maintenance on a specific diet. The albumin‑to‑creatinine ratio (ACR) was determined at 5 weeks and at 16 weeks, the kidneys were harvested, and morphological examination and immunohistochemical analysis were performed. The levels of malondialdehyde (MDA), as well as superoxide dismutase (SOD) and glutathione peroxidase (GSH‑px) activities in the kidneys were measured using appropriate assay kits. The expression of inflammatory factors and associated proteins was analyzed using western blot analysis. At 5 weeks, the levels of ACR in KK/HlJ mice were increased, which was inhibited by treatment with RHL. Treatment with RHL (50 mg/kg/day) decreased the body weight of KK/HlJ mice. Compared with the C57BL/J control, the KK/HlJ model mice had a significantly lower activity of SOD and GSH‑px in the kidneys, but had significantly higher levels of MDA. Treatment of KK/HlJ mice with RHL significantly increased the activities SOD and GSH‑px, and reduced the MAD level in the kidneys. Renal tubular epithelial cell edema was observed in KK/HlJ mice but not in C57BL/J mice. RHL decreased the incidence of renal tubular epithelial cell edema and significantly decreased the expression of TNF‑α and IL‑6 as well as the expression and phosphorylation of NF‑κB in the kidneys. Therefore, DN is associated with the expression of inflammatory factors, renal tubular epithelial cell edema and renal dysfunction in KK/HlJ mice. RHL improves renal function by decreasing kidney inflammation.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Lin, Y., Zhen, Y., Wei, J., Wei, J., Dai, J., Gao, J. ... Hu, G. (2017). Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice. Experimental and Therapeutic Medicine, 14, 5801-5808. https://doi.org/10.3892/etm.2017.5283
MLA
Lin, Y., Zhen, Y., Wei, J., Wei, J., Dai, J., Gao, J., Li, K., Hu, G."Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice". Experimental and Therapeutic Medicine 14.6 (2017): 5801-5808.
Chicago
Lin, Y., Zhen, Y., Wei, J., Wei, J., Dai, J., Gao, J., Li, K., Hu, G."Rhein lysinate protects renal function in diabetic nephropathy of KK/HlJ mice". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5801-5808. https://doi.org/10.3892/etm.2017.5283