Open Access

Interferon‑α inhibits cell migration and invasion and induces the expression of antiviral proteins in Huh‑7 cells transfected with hepatitis B virus X gene‑expressing lentivirus

  • Authors:
    • Qian Yang
    • Xiao‑Peng Li
    • Yuan‑Bin Zhong
    • Tian‑Xin Xiang
    • Lun‑Li Zhang
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5288
  • Pages:5924-5930
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatitis B virus (HBV) X protein (HBx) serves an important role in HBV infection and the development of HBV‑related liver cancer. Interferon‑α (IFN‑α) is used to treat patients with HBV; however, the role of IFN‑α in the development of HBV‑related liver cancer remains unclear. The present study established a new HBV‑related liver cancer model (Huh‑7‑HBx) by transfecting the hepatoma cell line Huh‑7, with HBx‑expressing lentivirus. Following IFN‑α treatment, cell viability, migration and invasion, as well as the expression of antiviral proteins in Huh‑7‑HBx, were subsequently determined. The results demonstrated that HBx‑expressing lentivirus had no significant effect on cell viability but promoted the migration and invasion of Huh‑7 cells. The expression of the antiviral genes IFN α and β receptor subunit 1 (IFNAR1), IFNAR2, IFN‑stimulated gene factor 3, double‑stranded RNA‑activated protein kinase and ribonuclease L, was also increased. Following treatment of Huh‑7‑HBx cells with IFN‑α, the expression of antiviral genes was increased at the level of transcription and translation, whereas cell migration and invasion was decreased. The present study suggests that IFN‑α may attenuate the development of HBV‑related liver cancer by reducing cell migration and invasion and promoting the expression of antiviral proteins.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Yang, Q., Li, X., Zhong, Y., Xiang, T., & Zhang, L. (2017). Interferon‑α inhibits cell migration and invasion and induces the expression of antiviral proteins in Huh‑7 cells transfected with hepatitis B virus X gene‑expressing lentivirus. Experimental and Therapeutic Medicine, 14, 5924-5930. https://doi.org/10.3892/etm.2017.5288
MLA
Yang, Q., Li, X., Zhong, Y., Xiang, T., Zhang, L."Interferon‑α inhibits cell migration and invasion and induces the expression of antiviral proteins in Huh‑7 cells transfected with hepatitis B virus X gene‑expressing lentivirus". Experimental and Therapeutic Medicine 14.6 (2017): 5924-5930.
Chicago
Yang, Q., Li, X., Zhong, Y., Xiang, T., Zhang, L."Interferon‑α inhibits cell migration and invasion and induces the expression of antiviral proteins in Huh‑7 cells transfected with hepatitis B virus X gene‑expressing lentivirus". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5924-5930. https://doi.org/10.3892/etm.2017.5288