Open Access

Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital

  • Authors:
    • Emmanuel Moustos
    • Dimitra Staphylaki
    • Athanasia Christidou
    • Demetrios A. Spandidos
    • Ioannis K. Neonakis
  • View Affiliations

  • Published online on: October 11, 2017     https://doi.org/10.3892/etm.2017.5291
  • Pages:6074-6076
  • Copyright: © Moustos et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The knowledge of the expected time-to-positivity (TTP) of blood cultures by major pathogens is essential both clinically and economically. To this end, we conducted the present two‑year study in our Institution, aiming to assess the TTP of all the major microorganisms including Enterobacteriaceae, Pseudomonas aeruginosa, Acinetoacter baumannii, Enterococcii spp, Staphylococcus aureus and yeasts, to determine whether a 3‑day interval is sufficient for their detection. The TTP for each case of strain isolation per patient was determined as the TTP of the first bottle among a set of bottles collected within the same period of time to be flagged as positive per patient. Based on our results, almost all major Gram‑negative (99.30%), Gram‑positive microbia (99.01%) and yeasts (98.85%) were detected within the first 5‑days of incubation, leading to the solid conclusion that a 5‑day period of incubation is adequate to detect almost all the major routine pathogens. By contrast, when a 3‑day period was examined acceptable results were only found for Gram‑negative (98.33%) and Gram‑positive (98.51%) microbia. A significant proportion of yeasts (8.05%) could not be detected within this time frame. Therefore, regarding the yeasts, a 3-day incubation period cannot be considered as adequate and is not advocated.

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December 2017
Volume 14 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Moustos, E., Staphylaki, D., Christidou, A., Spandidos, D.A., & Neonakis, I.K. (2017). Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital. Experimental and Therapeutic Medicine, 14, 6074-6076. https://doi.org/10.3892/etm.2017.5291
MLA
Moustos, E., Staphylaki, D., Christidou, A., Spandidos, D. A., Neonakis, I. K."Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital". Experimental and Therapeutic Medicine 14.6 (2017): 6074-6076.
Chicago
Moustos, E., Staphylaki, D., Christidou, A., Spandidos, D. A., Neonakis, I. K."Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6074-6076. https://doi.org/10.3892/etm.2017.5291