HMGB1, TGF‑β and NF‑κB are associated with chronic allograft nephropathy
- Shi‑Qi Zhao
- Zhen‑Zhen Xue
- Ling‑Zhang Wang
Published online on: October 17, 2017
The present study aimed to investigate the association between high mobility group protein B1 (HMGB1), transforming growth factor‑β1 (TGF‑β1), nuclear factor‑κB (NF‑κB) and chronic allograft nephropathy (CAN) and to identify the clinical significance of HMGB1, TGF‑β1, NF‑κB on patients with CAN. Between September 2012 and November 2014, 27 patients with CAN diagnosed by biopsy were enrolled in the present study and a further 30 patients that underwent nephrectomy following trauma were selected as the control group. Immunohistochemical staining with HMGB1, TGF‑β1 and NF‑κB expression in the renal tissues, and western blot analysis were used to measure the relative expression of HMGB1, TGF‑β1 and NF‑κB. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to estimate the relative expression of HMGB1, TGF‑β1 and NF‑κB mRNA. Statistical analysis was used to calculate the association between HMGB1, TGF‑β1 and NF‑κB expression and CAN grade. Immunohistochemical staining demonstrated that HMGB1, TGF‑β1 and NF‑κB had markedly positive expression rates in renal tubular epithelial cell cytoplasm and membranes in CAN renal tissues, and the positive rates of HMGB1, TGF‑β1 and NF‑κB increased with the aggravation of CAN pathological grade (I, II and III). The results of western blot analysis indicated that the expression levels of HMGB1, TGF‑β1 and NF‑κB were significantly higher in the CAN group, compared with the normal group (P<0.05), and the expression levels increased with the progression of CAN grade. A positive association among HMGB1, TGF‑β1 and NF‑κB expression was identified. RT‑qPCR analysis demonstrated that the expression of HMGB1, TGF‑β1 and NF‑κB mRNA in the CAN group was significantly higher than in the normal group (P<0.05), and the relative expression level of HMGB1, TGF‑β1 and NF‑κB mRNA not only increased with the aggravation of CAN grade, but was also positively associated with the expression of HMGB1, TGF‑β1 and NF‑κB, respectively. The abnormal expression of HMGB1, TGF‑β1 and NF‑κB is therefore, an important manifestation of CAN and the expression of HMGB1, TGF‑β1 and NF‑κB mRNA in the renal tissues are significantly associated with CAN pathological progression. HMGB1, TGF‑β1 and NF‑κB may form a signaling pathway that leads to the occurrence of CAN, which induces renal interstitial fibrosis.