(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

  • Authors:
    • Sufang Liu
    • Hongde Li
    • Min Tang
    • Ya Cao
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5495
  • Pages:1105-1112
Metrics: HTML 0 views | PDF 0 views
0

Abstract

The Epstein‑Barr virus (EBV) lytic cycle contributes to the development of EBV‑associated diseases. EBV‑encoded latent membrane protein 1 (LMP1) is key to EBV lytic replication, and our previous work indicated that epigallocatechin‑3‑gallate (EGCG) inhibited constitutive EBV lytic infection through the suppression of LMP1‑activated phosphoinositide 3‑kinase/Akt and mitogen‑activated protein kinase kinase/extracellular signal‑related protein kinase 1/2 signaling. The present study demonstrated that LMP1 in CNE‑LMP1 constructed cells significantly induced the expression of the EBV lytic proteins BZLF1 (P<0.001) and BMRF1 (P<0.05) compared with CNE1 cells. Following treatment with a specific DNAzyme that targets LMP1, significantly reduced protein expression levels of BZLF1 and BMRF1 in EBV‑associated epithelial carcinoma CNE1‑LMP1 cells (P<0.001 and P<0.01, respectively) and lymphoma B95.8 cells (both P<0.01) were observed. Furthermore, EGCG significantly inhibited the mRNA and protein expression levels of LMP1 (P<0.05) in an apparent dose‑dependent manner in CNE1‑LMP1 and B95.8 cells. Thus, the present findings indicated that the molecular mechanism underlying EGCG inhibition of EBV lytic infection involves downregulation of LMP1.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Liu, S., Li, H., Tang, M., & Cao, Y. (2018). (-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1. Experimental and Therapeutic Medicine, 15, 1105-1112. https://doi.org/10.3892/etm.2017.5495
MLA
Liu, S., Li, H., Tang, M., Cao, Y."(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1". Experimental and Therapeutic Medicine 15.1 (2018): 1105-1112.
Chicago
Liu, S., Li, H., Tang, M., Cao, Y."(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1105-1112. https://doi.org/10.3892/etm.2017.5495