Open Access

Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism

  • Authors:
    • Ying Qin
    • Shutong Zhuang
    • Jianfeng Wen
    • Kai Zheng
  • View Affiliations

  • Published online on: October 24, 2017     https://doi.org/10.3892/etm.2017.5370
  • Pages:191-197
  • Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer is the third leading cause of cancer‑associated mortality worldwide and is one of the most common malignancies in China. However, the molecular mechanisms underlying the tumorigenesis of gastric cancer remain largely unclear. Long non‑coding (Lnc)RNAs have been demonstrated to serve significant roles in the tumorigenesis of various types of cancer. The present study aimed to explore the role of the LncRNA mediator of DNA damage checkpoint protein 1‑antisense RNA (MDC1‑AS), the antisense transcript of MDC1, in human gastric cancer. The results revealed that the expression of MDC1‑AS in human gastric cancer was significantly suppressed in vivo and in vitro. In addition, overexpression of MDC1‑AS in the poorly differentiated gastric cancer cell line MKN28 significantly inhibited cell prolife­ration and metastasis, while the knockdown of MDC1‑AS in well‑differentiated MKN45 gastric cancer cells significantly increased proliferation and metastasis. The knockdown of MDC1 relieved the inhibitory effect of MDC1‑AS on MKN28 cell proliferation and metastasis, while the overexpression of MDC1 attenuated the stimulatory effect of MDC1‑AS knockdown in MKN45 cells. Thus, the present study demonstrated that MDC1‑AS had an inhibitory on gastric tumorigenesis through an MDC1‑dependent mechanism. This indicates that MDC1‑AS is a potential novel therapeutic target for the diagnosis and treatment of human gastric cancer in the clinic.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Qin, Y., Zhuang, S., Wen, J., & Zheng, K. (2018). Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism. Experimental and Therapeutic Medicine, 15, 191-197. https://doi.org/10.3892/etm.2017.5370
MLA
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15.1 (2018): 191-197.
Chicago
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15, no. 1 (2018): 191-197. https://doi.org/10.3892/etm.2017.5370