Open Access

The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes

  • Authors:
    • Zhaojin Du
    • Zhilei Qiu
    • Zhankun Wang
    • Xinsheng Wang
  • View Affiliations

  • Published online on: October 24, 2017     https://doi.org/10.3892/etm.2017.5359
  • Pages:305-309
  • Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to investigate the inhibitory effects of soybean isoflavones (SI) on testicular cell apoptosis in mice with type-2 diabetes, as well as any possible mechanisms of action. Thirty male C57BL/6J mice were randomly divided into the control, diabetic (model), and treatment (SI) groups (n=10 each). After treatment for 20 weeks, testicular cell apoptosis was detected and evaluated using DAPI staining. The expression and distribution of caspase-3 protein in testicular tissues was detected via immunohistochemistry, while caspase-3 mRNA expression was detected using RT-PCR. Bax and Bcl-2 protein expression was detected by western blot analysis. At week 20, DAPI staining showed that SI treatment significantly decreased testicular tissue cell apoptosis in diabetic mice. Immunohistochemical staining revealed that caspase-3 expression in the SI group was significantly reduced relative to the model group. RT-PCR showed that SI treatment significantly decreased caspase-3 mRNA expression relative to the model group. Western blot analysis revealed that SI treatment significantly decreased Bax protein expression and increased Bcl-2 protein expression (P<0.01). SI exhibited an inhibitory effect on testicular tissue cell apoptosis in mice with type 2 diabetes, with this effect possibly mediated by a decreased expression of caspase-3 and Bax and increased Bcl-2 protein expression.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Du, Z., Qiu, Z., Wang, Z., & Wang, X. (2018). The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes. Experimental and Therapeutic Medicine, 15, 305-309. https://doi.org/10.3892/etm.2017.5359
MLA
Du, Z., Qiu, Z., Wang, Z., Wang, X."The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes". Experimental and Therapeutic Medicine 15.1 (2018): 305-309.
Chicago
Du, Z., Qiu, Z., Wang, Z., Wang, X."The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes". Experimental and Therapeutic Medicine 15, no. 1 (2018): 305-309. https://doi.org/10.3892/etm.2017.5359