Open Access

Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells

  • Authors:
    • Fafeng Cheng
    • Chongyang Ma
    • Liangming Sun
    • Xiaoyu Zhang
    • Changming Zhai
    • Changxiang Li
    • Shuang Zhang
    • Beida Ren
    • Shuling Liu
    • Songnan Liu
    • Xiangjun Yin
    • Xueqian Wang
    • Qingguo Wang
  • View Affiliations

  • Published online on: October 30, 2017     https://doi.org/10.3892/etm.2017.5395
  • Pages:320-326
  • Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Endoplasmic reticulum stress (ERS) and autophagy activation play important roles in the process of cerebral ischemia/reperfusion (I/R) injury. The synergistic protective effects of Geniposide and ursodeoxycholic acid against cellular apoptosis caused by oxygen‑glucose deprivation‑reoxygenation (OGD/R) were investigated using a Cell Counting Kit‑8 assay, lactate dehydrogenase (LDH) assay, flow cytometry, quantitative polymerase chain reaction (qPCR), and western blotting to examine cellular viability, apoptosis, reactive oxygen species (ROS) levels, mRNA and protein levels, respectively, in relation to ERS and autophagy. We found that pretreatment with Geniposide improved cellular viability. Moreover, treatment with a combination of Geniposide and Tauroursodeoxycholic acid (TUDCA) (GT) protected injured cells better than Geniposide alone. Further studies showed that the increase in cellular ROS levels, and the overexpression of mRNA and proteins related to OGD/R‑induced ERS and autophagy, were both counteracted by GT. Our study indicates that the protective effects of GT on OGD/R‑induced apoptosis in SH‑SY5Y cells are associated with the inhibition of ERS and autophagy.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C. ... Wang, Q. (2018). Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells. Experimental and Therapeutic Medicine, 15, 320-326. https://doi.org/10.3892/etm.2017.5395
MLA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15.1 (2018): 320-326.
Chicago
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 320-326. https://doi.org/10.3892/etm.2017.5395