MicroRNA‑24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin‑α3 and regulating inflammatory responses

  • Authors:
    • Ye Zheng
    • Yongxing Li
    • Gang Liu
    • Xiangqian Qi
    • Xufen Cao
  • View Affiliations

  • Published online on: October 23, 2017     https://doi.org/10.3892/etm.2017.5355
  • Pages:338-344
Metrics: HTML 0 views | PDF 0 views
0

Abstract

The aim of the present study was to measure the level of microRNA (miRNA or miR)‑24 in the serum of patients with atherosclerosis and to investigate the effect of miR‑24 on the expression of importin‑α3 and tumor necrosis factor (TNF)‑α, as well as the proliferation and migration of vascular endothelial cells. A total of 30 patients with atherosclerosis admitted to hospital between January and June 2016 were enrolled in the present study; 30 healthy subjects with a similar age range were enrolled as controls. Peripheral blood (10 ml) was collected from all participants. Human umbilical vein endothelial cells (HUVECs) were transfected with miR‑24 mimic using Lipofectamine 2000. TargetScan was used to elucidate whether importin‑α3 (KPNA4) was a target gene of miR‑24. Expression levels of miR‑24 and mRNAs were measured using reverse transcription‑quantitative polymerase chain reaction, and protein expression was determined using western blotting. Cell Counting Kit 8 assay was used to assess the proliferation of HUVECs, and a Transwell assay was performed to detect the migration of HUVECs. Expression of miR‑24 in peripheral blood from patients with atherosclerosis was significantly lower when compared with healthy subjects (P<0.05). Overexpression of miR‑24 was demonstrated to significantly inhibit the transcription and translation of the importin‑α3 gene (P<0.05) and negatively regulate the expression of endothelial inflammatory factor TNF‑α (P<0.05). Furthermore, overexpression of miR‑24 significantly inhibited the proliferation and migration of HUVECs (P<0.05), and miR‑24 knockdown significantly promoted these processes (P<0.05). The results of the present study suggest that miR‑24 exerts its effect in atherosclerosis by blocking the nuclear factor‑κB signaling pathway, regulating inflammation in endothelial cells, and inhibiting the proliferation and migration of vascular endothelial cells.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zheng, Y., Li, Y., Liu, G., Qi, X., & Cao, X. (2018). MicroRNA‑24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin‑α3 and regulating inflammatory responses. Experimental and Therapeutic Medicine, 15, 338-344. https://doi.org/10.3892/etm.2017.5355
MLA
Zheng, Y., Li, Y., Liu, G., Qi, X., Cao, X."MicroRNA‑24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin‑α3 and regulating inflammatory responses". Experimental and Therapeutic Medicine 15.1 (2018): 338-344.
Chicago
Zheng, Y., Li, Y., Liu, G., Qi, X., Cao, X."MicroRNA‑24 inhibits the proliferation and migration of endothelial cells in patients with atherosclerosis by targeting importin‑α3 and regulating inflammatory responses". Experimental and Therapeutic Medicine 15, no. 1 (2018): 338-344. https://doi.org/10.3892/etm.2017.5355