Association of long non‑coding RNA GAS5 and miR‑21 levels in CD4+ T cells with clinical features of systemic lupus erythematosus
- Qi‑Feng Suo
- Jun Sheng
- Fu‑Yong Qiang
- Zong‑Sheng Tang
- Ying‑Ying Yang
Published online on: November 1, 2017
The present study aimed to assess the expression of growth arrest‑specific 5 (GAS5) and microRNA (miR)‑21 in systemic lupus erythematosus (SLE), and attempted to explore their association with clinical features. CD4+ T cells were isolated from peripheral blood of healthy donors and SLE patients by magnetic‑activated cell sorting. GAS5 and miR‑21 expression levels in cluster of differentiation (CD)4+ T cells were measured by reverse‑transcription quantitative polymerase chain reaction. The results revealed that GAS5 and miR‑21 levels were significantly elevated in CD4+ T cells of patients with SLE compared with those in control subjects (P<0.05). Regarding clinical features, SLE patients with ulceration had higher GAS5 expression levels in CD4+ T cells than those without ulceration (P<0.05), and the expression of miR‑21 was significantly higher in CD4+ T cells of SLE patients with low levels of complement component 3 (C3) than in those with normal levels of complement C3 (P<0.05). In conclusion, GAS5 and miR‑21 in CD4+ T cells may serve as potential biomarkers for the diagnosis and monitoring of the progression of SLE.