Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes

  • Authors:
    • Xiaoliu Liu
    • Sufang Liu
    • Jian Lei
    • Lixin Zou
    • Le Xiao
    • Guangsen Zhang
  • View Affiliations

  • Published online on: October 30, 2017     https://doi.org/10.3892/etm.2017.5402
  • Pages:500-505
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Abstract

As a highly heterogeneous disease, the pathogenesis of myelodysplastic syndrome (MDS) has not been well defined. In the present study, human mutS homolog 2 (hMSH2) promoter methylation was detected with methylation‑specific polymerase chain reaction (PCR). The function of hMSH2 was analyzed by microsatellite instability (MSI) detection of BAT‑26, and hMSH2 expression was evaluated using reverse transcription‑quantitative PCR in 60 patients with MDS. The results revealed methylation of the hMSH2 promoter in 18 patients with MDS who have an overall prevalence of 30% (95% confidence interval, 18.4‑41.6%). Among the patients with hMSH2 methylation, 2 patients exhibited MSI. It was demonstrated that hMSH2 promoter methylation was increased in MDS with an increase in Revised International Prognostic Scoring System (IPSS‑R) risk, and patients with higher hMSH2 promoter methylation had shorter overall survival by Kaplan‑Meier analysis (P=0.011). In addition, it was also observed that decreased hMSH2 mRNA expression was associated with high IPSS‑R risk group (high/very high vs. intermediate, P=0.003), and hMSH2 mRNA expression in CD34 positive bone marrow cells was lower compared with that in CD34 negative cells of patients with MDS (P=0.029). Methylation of hMSH2 may be valuable for prognostic evaluation and progression prediction of MDS. Furthermore, hMSH2 may serve a key function in the pathogenesis and prognosis of MDS.

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January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., & Zhang, G. (2018). Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes. Experimental and Therapeutic Medicine, 15, 500-505. https://doi.org/10.3892/etm.2017.5402
MLA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15.1 (2018): 500-505.
Chicago
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15, no. 1 (2018): 500-505. https://doi.org/10.3892/etm.2017.5402