miRNA‑145 is associated with spontaneous hypertension by targeting SLC7A1

Wang,Yong; Jin,Liyan

doi:10.3892/etm.2017.5371

miRNA‑145 is associated with spontaneous hypertension by targeting SLC7A1

Authors:
- Yong Wang
- Liyan Jin
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Affiliations: Department of General Practice, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Cardiology, The Second Affiliated Hospital of Henan College of Traditional Chinese Medicine, Zhengzhou, Henan 450002, P.R. China
Published online on: October 24, 2017 https://doi.org/10.3892/etm.2017.5371
Pages: 548-552

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Abstract

Previous studies have indicated that microRNAs (miRNAs/miRs) may participate in the pathogenesis of hypertension. miR‑145 has been demonstrated to serve important roles in the development of numerous cardiovascular diseases. However, the specific role of miR‑145 in hypertension remains unclear. The present study aimed to investigate the role of miR‑145 in spontaneously hypertensive rats (SHR) and rat vascular endothelial cells (RVECs). The results of the present study demonstrated that in the SHR group miR‑145 expression was significantly upregulated in the thoracic aorta compared with the control group. Furthermore, a significant decrease in nitric oxide (NO) content was observed in the SHR group compared with the control rats. In RVECs, silencing miR‑145 induced a significant increase in the expression of solute carrier family 7 member 1 (SLC7A1) and phosphorylated endothelial nitric oxide synthase, and a dual‑luciferase reporter assay confirmed that SLC7A1 is a direct target of miR‑145. The results of the present study indicate that miR‑145 functions as a key mediator in the pathogenesis of hypertension via targeting SLC7A1, which suggests that miR‑145 is a potential target for the treatment of hypertension.

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