Open Access

Radiosensitivity of human ovarian cancer cells is enhanced by pseudolaric acid B due to the inhibition of the Ras/Raf/ERK signaling pathway

  • Authors:
    • Quqing Song
    • Sheng Jiang
    • Xinxin Zhang
    • Chunxia Pan
    • Chunhua Lu
    • Jingwei Peng
    • Qingshui Li
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5500
  • Pages:685-690
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Ovarian cancer has the highest mortality rate among gynecological cancers; the most effective therapy for this cancer is a combination of radiation treatment and chemotherapy. However, radiation resistance is the leading factor associated with treatment failure. The present study aimed to investigate pseudolaric acid B (PAB) as a potential radiosensitizer for the treatment of ovarian cancer. The present study performed MTT and clonogenic assays, and demonstrated that PAB could induce a radiosensitizing effect on SKOV‑3 cells. An Annexin V/propidium iodide staining assay revealed that PAB exerted a radiosensitizing effect by inducing SKOV‑3 cell apoptosis. In addition, western blot analysis demonstrated that the activity of the Ras/RAF proto‑oncogene serine/threonine‑protein kinase/extracellular signal‑regulated kinase signaling pathway was reduced by combination therapy with PAB and irradiation. In conclusion, the present study establishes PAB as a radiosensitizer, and provides a rational basis for the use of PAB and irradiation as a combination therapy to treat ovarian cancer.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Song, Q., Jiang, S., Zhang, X., Pan, C., Lu, C., Peng, J., & Li, Q. (2018). Radiosensitivity of human ovarian cancer cells is enhanced by pseudolaric acid B due to the inhibition of the Ras/Raf/ERK signaling pathway. Experimental and Therapeutic Medicine, 15, 685-690. https://doi.org/10.3892/etm.2017.5500
MLA
Song, Q., Jiang, S., Zhang, X., Pan, C., Lu, C., Peng, J., Li, Q."Radiosensitivity of human ovarian cancer cells is enhanced by pseudolaric acid B due to the inhibition of the Ras/Raf/ERK signaling pathway". Experimental and Therapeutic Medicine 15.1 (2018): 685-690.
Chicago
Song, Q., Jiang, S., Zhang, X., Pan, C., Lu, C., Peng, J., Li, Q."Radiosensitivity of human ovarian cancer cells is enhanced by pseudolaric acid B due to the inhibition of the Ras/Raf/ERK signaling pathway". Experimental and Therapeutic Medicine 15, no. 1 (2018): 685-690. https://doi.org/10.3892/etm.2017.5500