Radiosensitivity of human ovarian cancer cells is enhanced by pseudolaric acid B due to the inhibition of the Ras/Raf/ERK signaling pathway
- Quqing Song
- Sheng Jiang
- Xinxin Zhang
- Chunxia Pan
- Chunhua Lu
- Jingwei Peng
- Qingshui Li
Published online on: November 13, 2017
Copyright: © Song et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
Ovarian cancer has the highest mortality rate among gynecological cancers; the most effective therapy for this cancer is a combination of radiation treatment and chemotherapy. However, radiation resistance is the leading factor associated with treatment failure. The present study aimed to investigate pseudolaric acid B (PAB) as a potential radiosensitizer for the treatment of ovarian cancer. The present study performed MTT and clonogenic assays, and demonstrated that PAB could induce a radiosensitizing effect on SKOV‑3 cells. An Annexin V/propidium iodide staining assay revealed that PAB exerted a radiosensitizing effect by inducing SKOV‑3 cell apoptosis. In addition, western blot analysis demonstrated that the activity of the Ras/RAF proto‑oncogene serine/threonine‑protein kinase/extracellular signal‑regulated kinase signaling pathway was reduced by combination therapy with PAB and irradiation. In conclusion, the present study establishes PAB as a radiosensitizer, and provides a rational basis for the use of PAB and irradiation as a combination therapy to treat ovarian cancer.