Open Access

Intraglandular transplantation of adipose‑derived stem cells combined with platelet‑rich fibrin extract for the treatment of irradiation‑induced salivary gland damage

  • Authors:
    • Zhifa Wang
    • Hongyan Xing
    • Hanqing Hu
    • Taiqiang Dai
    • Yan Wang
    • Zhijin Li
    • Ran An
    • Haiyan Xu
    • Yanpu Liu
    • Bin Liu
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5497
  • Pages:795-805
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

The aim of the present study was to determine the effect of adipose‑derived mesenchymal stem cells (ADSCs) combined with heterologous platelet‑rich fibrin extract (PRFe) on irradiation‑induced salivary gland (SG) damage. ADSCs were isolated from C3H mice, whereas PRFe was obtained from New Zealand rabbits. Twelve weeks post irradiation, the ADSCs or PRFe or their combination were transplanted into the submandibular glands of C3H mice with irradiation‑induced damage. The salivary flow rate (SFR) was determined and histopathological analysis was performed at 12 weeks post transplantation. Immunofluorescence, microvessel density measurements and transmission electron microscopy were performed to assess α‑amylase (AMY) production, apoptosis and microstructural changes in the cells. The administration of ADSCs combined with PRFe increased the SFR at 12 weeks post transplantation, whereas ADSCs alone or PRFe alone failed to do so. The ADSCs+PRFe‑treated, irradiated SGs had fewer damaged and atrophied acinar cells, higher AMY levels and an increased microvessel density compared with the untreated irradiated SGs. Moreover, SG tissue from the ADSCs+PRFe group also showed decreased apoptotic and increased proliferative activity compared to that from the irradiated group. In conclusion, ADSCs or PRFe alone did not restore permanent, irradiation‑induced damage of SG tissue when used alone, but when used together, they provided effective treatment outcomes.

Related Articles

Journal Cover

January 2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wang, Z., Xing, H., Hu, H., Dai, T., Wang, Y., Li, Z. ... Liu, B. (2018). Intraglandular transplantation of adipose‑derived stem cells combined with platelet‑rich fibrin extract for the treatment of irradiation‑induced salivary gland damage. Experimental and Therapeutic Medicine, 15, 795-805. https://doi.org/10.3892/etm.2017.5497
MLA
Wang, Z., Xing, H., Hu, H., Dai, T., Wang, Y., Li, Z., An, R., Xu, H., Liu, Y., Liu, B."Intraglandular transplantation of adipose‑derived stem cells combined with platelet‑rich fibrin extract for the treatment of irradiation‑induced salivary gland damage". Experimental and Therapeutic Medicine 15.1 (2018): 795-805.
Chicago
Wang, Z., Xing, H., Hu, H., Dai, T., Wang, Y., Li, Z., An, R., Xu, H., Liu, Y., Liu, B."Intraglandular transplantation of adipose‑derived stem cells combined with platelet‑rich fibrin extract for the treatment of irradiation‑induced salivary gland damage". Experimental and Therapeutic Medicine 15, no. 1 (2018): 795-805. https://doi.org/10.3892/etm.2017.5497