Open Access

Astragaloside protects myocardial cells from apoptosis through suppression of the TLR4/NF-κB signaling pathway

  • Authors:
    • Yang Zhao
    • Zhongfen Liu
    • Hu Zhang
  • View Affiliations

  • Published online on: November 21, 2017     https://doi.org/10.3892/etm.2017.5535
  • Pages: 1505-1509
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Astragaloside is a monomer isolated from Astragalus membranaceus, a flowering plant in the family Fabaceae. The aim of the present study was to investigate the anti-apoptotic affect of astragaloside on myocardial cells through the TLR4/NF-κB signaling pathway. Astragaloside, NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC) and Toll-like receptor 4 (TLR4) blocking antibody solution were prepared in vitro, and myocardial cells were incubated and cultured in serum-free medium overnight. Cells were divided into five groups: the normal control group, serum-free group, astragaloside group, TLR4 blocking antibody group and NF-κB inhibitor PDTC group. The myocardial cell apoptosis in each group was detected using flow cytometry, and the expression levels of TLR4 and NF-κB were detected via western blotting. The apoptosis rate in the serum-free group was significantly higher than that in the normal control group. The apoptosis rate of myocardial cells in the TLR4 blocking antibody group and NF-κB inhibitor PDTC group was lower than that in the serum-free group. In addition, the myocardial cell apoptosis was more obviously decreased in the astragaloside group, and the protein expression levels of TLR4 and NF-κB in the serum-free group were significantly higher than those in normal control group. The protein expression levels of TLR4 and NF-κB in the astragaloside group were obviously lower than those in the serum-free group, and the protein expression levels of TLR4 and NF-κB in the TLR4 blocking antibody group and NF-κB inhibitor PDTC group were decreased. In conclusion, astragaloside reduced myocardial cell apoptosis and protected myocardial cells, which may be one of the mechanisms of a traditional Chinese medicine monomer in treating heart failure.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Zhao, Y., Liu, Z., & Zhang, H. (2018). Astragaloside protects myocardial cells from apoptosis through suppression of the TLR4/NF-κB signaling pathway. Experimental and Therapeutic Medicine, 15, 1505-1509. https://doi.org/10.3892/etm.2017.5535
MLA
Zhao, Y., Liu, Z., Zhang, H."Astragaloside protects myocardial cells from apoptosis through suppression of the TLR4/NF-κB signaling pathway". Experimental and Therapeutic Medicine 15.2 (2018): 1505-1509.
Chicago
Zhao, Y., Liu, Z., Zhang, H."Astragaloside protects myocardial cells from apoptosis through suppression of the TLR4/NF-κB signaling pathway". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1505-1509. https://doi.org/10.3892/etm.2017.5535