Resveratrol protects neuronal cells from isoflurane‑induced inflammation and oxidative stress‑associated death by attenuating apoptosis via Akt/p38 MAPK signaling

  • Authors:
    • Weilan Hu
    • Ei Yang
    • Jianxin Ye
    • Weili Han
    • Zeng‑Li Du
  • View Affiliations

  • Published online on: November 17, 2017     https://doi.org/10.3892/etm.2017.5527
  • Pages:1568-1573
Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

The aim of the present study was to determine whether resveratrol protects neuronal cells from inflammation and isoflurane‑induced oxidative stress‑associated death via attenuating apoptosis via Akt/p38 mitogen‑activated protein kinase (MAPK) signaling. The PC12 rat pheochromocytoma cell line was treated with 2% isoflurane + 21% O2 + 5% CO2 for 6 h and pre‑treated with resveratrol (0‑1,000 µM) for 0, 24 or 48 h prior to isoflurane treatment. An MTT assay, flow cytometry and ELISA of tumor necrosis factor‑α, interleukin‑6, malondialdehyde and superoxide dismutase revealed that resveratrol reduced growth inhibition, restrained apoptosis and suppressed inflammation and oxidative stress induced by isoflurane in PC12 cells. Pretreatment with resveratrol effectively reduced caspase‑3 activity and inducible nitric oxide synthase protein expression in isoflurane‑induced PC12 cells. In addition, western blot analysis demonstrated that resveratrol treatment significantly attenuated isoflurane‑induced decreases in the activated phosphorylated (p)‑Akt/Akt ratio and increases in the p‑p38/p38 MAPK protein ratio in PC12 cells. These findings indicated that resveratrol was able to protect neuronal cells from isoflurane‑induced inflammation and oxidative stress‑associated death by attenuating apoptosis via Akt/p38 MAPK signaling.

Related Articles

Journal Cover

February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Hu, W., Yang, E., Ye, J., Han, W., & Du, Z. (2018). Resveratrol protects neuronal cells from isoflurane‑induced inflammation and oxidative stress‑associated death by attenuating apoptosis via Akt/p38 MAPK signaling. Experimental and Therapeutic Medicine, 15, 1568-1573. https://doi.org/10.3892/etm.2017.5527
MLA
Hu, W., Yang, E., Ye, J., Han, W., Du, Z."Resveratrol protects neuronal cells from isoflurane‑induced inflammation and oxidative stress‑associated death by attenuating apoptosis via Akt/p38 MAPK signaling". Experimental and Therapeutic Medicine 15.2 (2018): 1568-1573.
Chicago
Hu, W., Yang, E., Ye, J., Han, W., Du, Z."Resveratrol protects neuronal cells from isoflurane‑induced inflammation and oxidative stress‑associated death by attenuating apoptosis via Akt/p38 MAPK signaling". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1568-1573. https://doi.org/10.3892/etm.2017.5527