Tubeimoside I attenuates inflammation and oxidative damage in a mice model of PM2.5‑induced pulmonary injury

  • Authors:
    • Jin‑Bo Zhang
    • Lei Zhang
    • Shi‑Qing Li
    • Ai‑Hua Hou
    • Wei‑Chao Liu
    • Ling‑Ling Dai
  • View Affiliations

  • Published online on: December 5, 2017     https://doi.org/10.3892/etm.2017.5597
  • Pages:1602-1607
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Abstract

In the present study, the effects of tubeimoside I (TBMS1) on particulate matter <2.5 µm in diameter (PM2.5)‑induced pulmonary injury and its mechanisms of action were investigated. Male BALB/c mice were randomly assigned into five groups (n=10/group): Control, PM2.5, PM2.5 + TBMS1 45 mg/kg, PM2.5 + TBMS1 90 mg/kg and PM2.5 + TBMS1 180 mg/kg. The dose of the PM2.5 suspension administered to the mice was 40 mg/kg via nasal instillation. The PM2.5 + TBMS1 groups received TBMS1 daily orally for 21 consecutive days, while the mice in the control and PM2.5 groups received equivalent volumes of PBS. Subsequently, lactic dehydrogenase, acid phosphatase, alkaline phosphatase, albumin, tumor necrosis factor‑α and interleukin‑6 protein levels in bronchoalveolar lavage fluid were determined. Oxidative stress was evaluated by detecting the protein levels of malondialdehyde, superoxide dismutase and inducible nitric oxide synthase, and the level of nitric oxide in lung tissue. Lastly, histopathological images of lung sections were obtained to observe changes in the lung tissue with treatment. The results indicated that exposure to PM2.5 induced pathological pulmonary changes, and biofilm and parenchymal cell damage, and promoted inflammation and oxidative stress. Treatment with TBMS1 attenuated the development of PM2.5‑induced pulmonary injury. Its mechanisms of action were associated with reducing cytotoxic effects, levels of inflammatory mediators and oxidative damage. In conclusion, the results of the present study indicate that TBMS1 is a potential therapeutic drug for treating PM2.5‑induced pulmonary injury.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhang, J., Zhang, L., Li, S., Hou, A., Liu, W., & Dai, L. (2018). Tubeimoside I attenuates inflammation and oxidative damage in a mice model of PM2.5‑induced pulmonary injury. Experimental and Therapeutic Medicine, 15, 1602-1607. https://doi.org/10.3892/etm.2017.5597
MLA
Zhang, J., Zhang, L., Li, S., Hou, A., Liu, W., Dai, L."Tubeimoside I attenuates inflammation and oxidative damage in a mice model of PM2.5‑induced pulmonary injury". Experimental and Therapeutic Medicine 15.2 (2018): 1602-1607.
Chicago
Zhang, J., Zhang, L., Li, S., Hou, A., Liu, W., Dai, L."Tubeimoside I attenuates inflammation and oxidative damage in a mice model of PM2.5‑induced pulmonary injury". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1602-1607. https://doi.org/10.3892/etm.2017.5597