MicroRNA‑30a suppresses non‑small‑cell lung cancer by targeting Myb‑related protein B

  • Authors:
    • Guo‑Jun Geng
    • Ying‑Tao Yang
    • Jie Jiang
    • Xiu‑Yi Yu
    • Xian‑En Fa
  • View Affiliations

  • Published online on: November 24, 2017     https://doi.org/10.3892/etm.2017.5559
  • Pages: 1633-1639
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Abstract

Non‑small‑cell lung cancer (NSCLC) is one of the leading causes of cancer mortality worldwide. A growing body of evidence indicates that microRNA (miR) have important and diverse roles in the proliferation, apoptosis and metastasis of human cancer cells. In the present study, the molecular regulation mechanism of miR‑30a and its potential target, Myb‑related protein B (MYBL2) was investigated in NSCLC. Reverse transcription‑quantitative polymerase chain reaction results showed that miR‑30a was significantly downregulated in NSCLC tissues compared with adjacent normal tissues (P<0.05). MYBL2 has a putative miR‑30a target site in its 3'untranslated region according to previous data, prediction databases and TargetScan software. In the present study, a negative correlation was demonstrated between miR‑30a and MYBL2 expression in NSCLC. Direct interaction between miR‑30a and MYBL2 was also confirmed via a dual‑luciferase reporter assay. miR‑30a overexpression inhibited the growth of A549 and H460 cells via MTT and bromodeoxyuridine incorporation assays, whereas miR‑30a downregulation promoted cell proliferation. In addition, miR‑30a overexpression not only increased cell apoptosis and induced cell cycle arrest in A549 and H460 cell lines, but also attenuated tumor growth, and mRNA and protein expression levels of MYBL2. The present findings suggest that miR‑30a may suppress NSCLC by targeting MYBL2.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Geng, G., Yang, Y., Jiang, J., Yu, X., & Fa, X. (2018). MicroRNA‑30a suppresses non‑small‑cell lung cancer by targeting Myb‑related protein B. Experimental and Therapeutic Medicine, 15, 1633-1639. https://doi.org/10.3892/etm.2017.5559
MLA
Geng, G., Yang, Y., Jiang, J., Yu, X., Fa, X."MicroRNA‑30a suppresses non‑small‑cell lung cancer by targeting Myb‑related protein B". Experimental and Therapeutic Medicine 15.2 (2018): 1633-1639.
Chicago
Geng, G., Yang, Y., Jiang, J., Yu, X., Fa, X."MicroRNA‑30a suppresses non‑small‑cell lung cancer by targeting Myb‑related protein B". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1633-1639. https://doi.org/10.3892/etm.2017.5559