Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma

  • Authors:
    • Jianguang Jia
    • Yiyu Qin
    • Ligong Zhang
    • Chenxu Guo
    • Yaguo Wang
    • Xicheng Yue
    • Jun Qian
  • View Affiliations

  • Published online on: November 24, 2017     https://doi.org/10.3892/etm.2017.5560
  • Pages:1718-1727
Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Sijunzi decoction (SJZD) is a traditional Chinese herbal medicine. Previous studies have indicated that SJZD exhibits antitumor activity. However, the underlying molecular mechanism has not been fully elucidated. To explore the antitumor mechanism of SJZD, the effects of serum from rats treated with SJZD on the proliferation of MKN‑28 and HGC‑27 gastric carcinoma cell lines were systematically investigated. It was found that SJZD‑treated rat serum significantly inhibited the growth of MKN‑28 and HGC‑27 cells in vitro. The results obtained from a colony formation assay showed that SJZD‑treated rat serum decreased the colony formation ability of MKN‑28 and HGC‑27 cells. The apoptosis rate in MKN‑28 and HGC‑27 cells was also increased following treatment with SJZD‑treated rat serum. Flow cytometry with cell sorting revealed the presence of side population (SP) cells in MKN‑28 and HGC‑27 cells though Hoechst 33342 staining, and verapamil reduced the SP percentage. Further analysis showed that SJZD‑treated rat serum promoted the apoptosis of SP cells in MKN‑28 and HGC‑27 cell lines by upregulating Bax, caspase‑3 and PARP and downregulating bcl‑2. These data revealed the therapeutic effect of SJZD‑treated rat serum on gastric carcinoma. Following the preliminary identification of the inhibitory effect on the growth of gastric cancer cells in vitro, the growth inhibitory effect of SJZD‑treated rat serum on SP cells was confirmed, and this inhibition particularly involved the induction of cell apoptosis.

Related Articles

Journal Cover

February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Jia, J., Qin, Y., Zhang, L., Guo, C., Wang, Y., Yue, X., & Qian, J. (2018). Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma. Experimental and Therapeutic Medicine, 15, 1718-1727. https://doi.org/10.3892/etm.2017.5560
MLA
Jia, J., Qin, Y., Zhang, L., Guo, C., Wang, Y., Yue, X., Qian, J."Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma". Experimental and Therapeutic Medicine 15.2 (2018): 1718-1727.
Chicago
Jia, J., Qin, Y., Zhang, L., Guo, C., Wang, Y., Yue, X., Qian, J."Sijunzi decoction‑treated rat serum induces apoptosis of side population cells in gastric carcinoma". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1718-1727. https://doi.org/10.3892/etm.2017.5560