Open Access

Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy

  • Authors:
    • Meng Zhang
    • Dedong Xiong
    • Hongxia Li
    • Zonglan Wang
    • Renzhe Li
  • View Affiliations

  • Published online on: November 8, 2017     https://doi.org/10.3892/etm.2017.5464
  • Pages:1871-1874
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The purpose of the present study was to investigate the diagnostic value of T cells spot test (T-Spot TB) combined with interferon-γ (INF-γ) and interleukin-27 (IL-27) in tuberculous pleurisy. Sixty patients with tuberculous pleurisy (observation group) and 60 patients with non-tuberculous pleurisy (control group) were enrolled in this study. T-Spot TB was performed to detect the pleural effusion of two groups of patients. Levels of IFN-γ and IL-27 in serum and pleural effusion were detected by enzyme-linked immunosorbent assay (ELISA). Relative expression of IFN-γ mRNA and IL-27 mRNA in peripheral blood mononuclear cells were detected by RT-PCR. Positive rate of T-Spot TB in observation group was 96.7% (58 cases), which was significantly higher than that in control group (p<0.05). Concentration of INF-γ in pleural effusion of observation group was 468.6±24.8 ng/l, which was significantly higher than that in control group (131.3±18.7 ng/l, p<0.05). Concentration of IL-27 in pleural effusion of observation group was 423.4±37.2 ng/l, which was significantly higher than that in control group (116.2±15.5 ng/l, p<0.05). Concentrations of INF-γ and IL-27 in serum of observation group were 48.2±13.4 and 41.7±10.6 ng/l, respectively, which were significantly higher than those in control group (38.6±11.2 and 35.3±8.4 ng/l, p<0.05). Relative expression levels of INF-γ mRNA and IL-27 mRNA in observation group were significantly higher than those in control group (p<0.05). Therefore, combination of T-Spot TB with INF-γ and IL-27 has significant application value in the clinical diagnosis of tuberculous pleurisy, and should be popularized.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Zhang, M., Xiong, D., Li, H., Wang, Z., & Li, R. (2018). Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy. Experimental and Therapeutic Medicine, 15, 1871-1874. https://doi.org/10.3892/etm.2017.5464
MLA
Zhang, M., Xiong, D., Li, H., Wang, Z., Li, R."Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy". Experimental and Therapeutic Medicine 15.2 (2018): 1871-1874.
Chicago
Zhang, M., Xiong, D., Li, H., Wang, Z., Li, R."Diagnostic value of T-Spot TB combined with INF-γ and IL-27 in tuberculous pleurisy". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1871-1874. https://doi.org/10.3892/etm.2017.5464