Open Access

Development and identification of Set transgenic mice

  • Authors:
    • Siliang Xu
    • Xiaoqiang Liu
    • Lingling Gao
    • Boqun Xu
    • Jianmin Li
    • Chao Gao
    • Yugui Cui
    • Jiayin Liu
  • View Affiliations

  • Published online on: December 11, 2017     https://doi.org/10.3892/etm.2017.5612
  • Pages:1982-1988
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a multifunctional protein involved in numerous biological processes, Set is expressed in several embryonic and adult organs. Furthermore, Set is overexpressed in numerous types of human cancers, including acute myeloid leukemia, breast cancer and pancreatic cancer. The expression of Set in germ cells is involved in gonad development, and the overexpression of Set has been observed in polycystic ovaries. In order to elucidate the physiological and pathological roles of Set, a Set transgenic mouse model was developed, in which the global overexpression of Set in adult tissues could be induced via the Cre/loxP system with the precise deletion of the Stop fragment in double‑transgenic hybrids. This result was then confirmed by genotypical and protein analysis using polymerase chain reaction and bioluminescence imaging. In conclusion, the conditional Set transgenic mice carrying a reporter system were successfully generated. The transgenic mice open a new window for the further investigation of the function of Set using tissue‑specific Cre mice and inducible Cre systems.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Xu, S., Liu, X., Gao, L., Xu, B., Li, J., Gao, C. ... Liu, J. (2018). Development and identification of Set transgenic mice. Experimental and Therapeutic Medicine, 15, 1982-1988. https://doi.org/10.3892/etm.2017.5612
MLA
Xu, S., Liu, X., Gao, L., Xu, B., Li, J., Gao, C., Cui, Y., Liu, J."Development and identification of Set transgenic mice". Experimental and Therapeutic Medicine 15.2 (2018): 1982-1988.
Chicago
Xu, S., Liu, X., Gao, L., Xu, B., Li, J., Gao, C., Cui, Y., Liu, J."Development and identification of Set transgenic mice". Experimental and Therapeutic Medicine 15, no. 2 (2018): 1982-1988. https://doi.org/10.3892/etm.2017.5612