Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyte injury

  • Authors:
    • Qing Gao
    • Alhossain Sarkar
    • Yizhi Chen
    • Bo Xu
    • Xiaojuan Zhu
    • Yang Yuan
    • Tianjun Guan
  • View Affiliations

  • Published online on: December 15, 2017     https://doi.org/10.3892/etm.2017.5643
  • Pages:2054-2061
Metrics: HTML 0 views | PDF 0 views     Cited By: 0 citations

Abstract

Deregulated lipid metabolism is a characteristic of metabolic diseases including type 2 diabetes and obesity, and likely contributes to podocyte injury and end‑stage kidney disease. Heart‑type fatty acid binding protein (H‑FABP) was reported to be associated with lipid metabolism. The present study investigated whether H‑FABP contributes to podocyte homeostasis. Podocytes were transfected by lentiviral vector to construct a cell line which stably overexpressed H‑FABP. Small interfering RNA capable of effectively silencing H‑FABP was introduced into podocytes to construct a cell line with H‑FABP knockdown. Certain groups were treated with palmitic acid (PA) and the fat metabolism, as well as inflammatory and oxidative stress markers were measured. PA accelerated lipid metabolism derangement, inflammatory reaction and oxidative stress in podocytes. Overexpression of H‑FABP enhanced the PA‑induced disequilibrium in podocytes. The mRNA and protein expression levels of acyl‑coenzyme A oxidase 3 and monocyte chemotactic protein 1, and the protein expression levels of 8‑hydroxy‑2'‑deoxyguanosine and 4‑hydroxynonenal were upregulated in the H‑FABP overexpression group, while the mRNA and protein expression of peroxisome proliferator activated receptor α was downregulated. Knockdown of H‑FABP inhibited the PA‑induced injury and lipid metabolism derangement, as well as the inflammatory reaction and oxidative stress in podocytes. These results indicated that overexpression of H‑FABP enhances fatty acid‑induced podocyte injury, while H‑FABP inhibition may represent a potential therapeutic strategy for the prevention of lipid metabolism-associated podocyte injury.

Related Articles

Journal Cover

February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Gao, Q., Sarkar, A., Chen, Y., Xu, B., Zhu, X., Yuan, Y., & Guan, T. (2018). Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyte injury. Experimental and Therapeutic Medicine, 15, 2054-2061. https://doi.org/10.3892/etm.2017.5643
MLA
Gao, Q., Sarkar, A., Chen, Y., Xu, B., Zhu, X., Yuan, Y., Guan, T."Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyte injury". Experimental and Therapeutic Medicine 15.2 (2018): 2054-2061.
Chicago
Gao, Q., Sarkar, A., Chen, Y., Xu, B., Zhu, X., Yuan, Y., Guan, T."Overexpression of heart-type fatty acid binding protein enhances fatty acid-induced podocyte injury". Experimental and Therapeutic Medicine 15, no. 2 (2018): 2054-2061. https://doi.org/10.3892/etm.2017.5643