Tangshen formula improves inflammation in renal tissue of diabetic nephropathy through SIRT1/NF-κB pathway

  • Authors:
    • Yue‑Guang Du
    • Ke‑Na Zhang
    • Zong‑Lei Gao
    • Fengjiao Dai
    • Xi‑Xi Wu
    • Ke‑Fu Chai
  • View Affiliations

  • Published online on: December 12, 2017     https://doi.org/10.3892/etm.2017.5621
  • Pages:2156-2164
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Abstract

The present study investigated the mechanism underlying the anti‑inflammatory effects of Tangshen formula (TS) in Sprague Dawley (SD) rats with diabetic nephropathy (DN). A rat model of DN was established by intraperitoneal injection of 1% (40 mg/kg) streptozotocin and administration of a high fat and glucose diet. Subsequently, SD rats were randomly divided into six groups (n=8): A DN group, a valsartan group, a high‑dose TS group, a middle‑dose TS group, a low‑dose TS group and a control group with normal SD rats. Once rats received their allocated treatment for 12 weeks, body weight and kidney weight were recorded, and fasting blood glucose, ratio of urinary protein, β2‑MG and creatinine clearance rate were determined. Furthermore, hemodynamic indices, including plasma viscosity and whole blood reduction viscosity were detected. Immunohistochemistry was used to detect the infiltration of macrophages in the kidneys of rats. Reverse transcription‑quantitative polymerase chain reaction and western blotting were performed to investigate the activation; mRNA and protein expression levels of monocyte chemoattractant protein‑1 (MCP‑1), macrophage migration inhibitory factor (MIF), nuclear factor‑κB (NF‑κB) and sirtuin‑1 (SIRT1) in each group. In comparison with the DN group, each biochemical indicator of rats in the high‑dose TS group was significantly decreased (P<0.05). Blood viscosity in each treatment group was significantly decreased when compared with the DN group (P<0.01). Hematoxylin and eosin staining indicated that the infiltration of macrophages was significantly decreased in the high‑dose TS group when compared with the DN group (P<0.01). mRNA and protein expression levels of MCP‑1 and MIF in the high‑dose TS group were significantly decreased when compared with the DN group (P<0.05). In the treatment groups, SITR1 mRNA expression levels were significantly increased, whereas the mRNA expression levels of NF‑κB were significantly decreased (P<0.01). Western blotting results indicated a significant decrease in the protein expression levels of acetylated NF‑κB in the treatment groups when compared with the DN group (P<0.01) and the propensity of protein expression of the other inflammatory factors were consistent with the mRNA findings. The results of the high‑dose TS group were similar to those of the valsartan group. The present study indicates that TS was able to activate SITR1, which lead to NF‑κB deacetylation, thus reducing the release of inflammatory factors and decreasing the severity of diabetic nephropathy.

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February 2018
Volume 15 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
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APA
Du, Y., Zhang, K., Gao, Z., Dai, F., Wu, X., & Chai, K. (2018). Tangshen formula improves inflammation in renal tissue of diabetic nephropathy through SIRT1/NF-κB pathway. Experimental and Therapeutic Medicine, 15, 2156-2164. https://doi.org/10.3892/etm.2017.5621
MLA
Du, Y., Zhang, K., Gao, Z., Dai, F., Wu, X., Chai, K."Tangshen formula improves inflammation in renal tissue of diabetic nephropathy through SIRT1/NF-κB pathway". Experimental and Therapeutic Medicine 15.2 (2018): 2156-2164.
Chicago
Du, Y., Zhang, K., Gao, Z., Dai, F., Wu, X., Chai, K."Tangshen formula improves inflammation in renal tissue of diabetic nephropathy through SIRT1/NF-κB pathway". Experimental and Therapeutic Medicine 15, no. 2 (2018): 2156-2164. https://doi.org/10.3892/etm.2017.5621