MicroRNA-133b inhibits cell proliferation and promotes apoptosis by targeting cullin 4B in esophageal squamous cell carcinoma

Huang,Hairong; Xu,Yang; Guo,Zhong; Chen,Xi; Ji,Shaiguang; Xu,Zhiyun

doi:10.3892/etm.2018.5906

MicroRNA-133b inhibits cell proliferation and promotes apoptosis by targeting cullin 4B in esophageal squamous cell carcinoma

Authors:
- Hairong Huang
- Yang Xu
- Zhong Guo
- Xi Chen
- Shaiguang Ji
- Zhiyun Xu
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Affiliations: Department of Cardiothoracic Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210002, P.R. China, Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China
Published online on: February 28, 2018 https://doi.org/10.3892/etm.2018.5906
Pages: 3743-3750
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

microRNAs (miRs) serve a role as modulators during carcinogenesis. It has been demonstrated that the expression of miR‑133b is decreased in a variety of tumor tissues and cell lines and serves a suppressive role in the proliferation and apoptosis of different types of tumor cells. However, its effect on esophageal squamous cell carcinoma (ESCC) cells remains unclear. In the current study, the expression of mature miR‑133b was measured using reverse transcription-quantitative polymerase chain reaction and the results indicated that miR‑133b was significantly downregulated in ESCC tissues and various ESCC cell lines. The overexpression of miR‑133b significantly inhibited the proliferation and promoted the apoptosis of KYSE150 and Eca‑109 cells. Furthermore, it was demonstrated that cullin 4B (CUL4B) promotes ESCC cell proliferation and inhibits apoptosis by activating the protein kinase B/glycogen synthase kinase 3β/β‑catenin pathway. Taken together, these results demonstrate that miR‑133b/CUL4B serves a tumor suppressive role during ESCC progression and may therefore be used as a potential target to treat patients with ESCC.

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