β-Endorphin attenuates collagen-induced arthritis partially by inhibiting peripheral pro-inflammatory mediators

He,Xiaofen; Huang,Lei; Qiu,Shenchen; Yin,Xiaohu; Shen,Yafang; Wu,Yuanyuan; Jiang,Yongliang; Fang,Jianqiao

doi:10.3892/etm.2018.5914

β-Endorphin attenuates collagen-induced arthritis partially by inhibiting peripheral pro-inflammatory mediators

Authors:
- Xiaofen He
- Lei Huang
- Shenchen Qiu
- Xiaohu Yin
- Yafang Shen
- Yuanyuan Wu
- Yongliang Jiang
- Jianqiao Fang
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Affiliations: Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China
Published online on: February 28, 2018 https://doi.org/10.3892/etm.2018.5914
Pages: 4014-4018

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Abstract

The classical analgesic pathway of opioids by binding their receptors in the nervous system is well known. However, little is known regarding opioid analgesia through the anti‑inflammatory pathway. The present study aimed to investigate the analgesic and anti‑inflammatory effect of β‑endorphin on inflammatory pain. A rat model of collagen‑induced arthritis (CIA) was generated by intradermal injection of bovine type II collagen. Rats were divided into the CIA + saline group and the CIA + β‑endorphin group, in which rats were intraperitoneally injected with β‑endorphin once every other day from day 18 following the injection of CII until day 28. Thermal hyperalgesia as determined by tail flick latency (TFL), as well as paw arthritis index and swelling. Tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β and IL‑6 mRNA expression in synovial tissue and their protein levels in paw inflammatory tissue were measured. The rat CIA model was successfully induced as indicated by the significantly decreased TFL, increased paw arthritis index and percentage of swelling on day 18. β‑endorphin treatment significantly increased the TFL, while decreasing the paw arthritis index and swelling in CIA rats. It also significantly downregulated TNF‑α and IL‑1β mRNA expression in synovial tissue and their protein levels in inflammatory tissue of the paws of CIA rats, while it had no significant effect on the levels of IL‑6. These results indicated that β‑endorphin suppresses peripheral pro‑inflammatory mediators in collagen‑induced arthritis, which may contribute to its analgesic effect.

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1	Stein C: The control of pain in peripheral tissue by opioids. N Engl J Med. 332:1685–1690. 1995. View Article : Google Scholar : PubMed/NCBI
2	Stein C and Yassouridis A: Peripheral morphine analgesia. Pain. 71:119–121. 1997. View Article : Google Scholar : PubMed/NCBI
3	Machelska H, Cabot PJ, Mousa SA, Zhang Q and Stein C: Pain control in inflammation governed by selectins. Nat Med. 4:1425–1428. 1998. View Article : Google Scholar : PubMed/NCBI
4	Rittner HL, Machelska H and Stein C: Leukocytes in the regulation of pain and analgesia. J Leukoc Biol. 78:1215–1222. 2005. View Article : Google Scholar : PubMed/NCBI
5	Hale KD, Ghanta VK, Gauthier DK and Hiramoto RN: Effects of rotational stress of different duration on NK cell activity, proinflammatory cytokines, and POMC-derived peptides in mice. Neuroimmunomodulation. 9:34–40. 2001. View Article : Google Scholar : PubMed/NCBI
6	Mousa SA, Shakibaei M, Sitte N, Schäfer M and Stein C: Subcellular pathways of beta-endorphin synthesis, processing, and release from immunocytes in inflammatory pain. Endocrinology. 145:1331–1341. 2004. View Article : Google Scholar : PubMed/NCBI
7	Walker JS: Anti-inflammatory effects of opioids. Adv Exp Med Biol. 521:148–160. 2003.PubMed/NCBI
8	Lee DM and Weinblatt ME: Rheumatoid arthritis. Lancet. 358:903–911. 2001. View Article : Google Scholar : PubMed/NCBI
9	Brennan FM and McInnes IB: Evidence that cytokines play a role in rheumatoid arthritis. J Clin Invest. 118:3537–3545. 2008. View Article : Google Scholar : PubMed/NCBI
10	Stein C, Comisel K, Haimerl E, Yassouridis A, Lehrberger K, Herz A and Peter K: Analgesic effect of intraarticular morphine after arthroscopic knee surgery. N Engl J Med. 325:1123–1126. 1991. View Article : Google Scholar : PubMed/NCBI
11	Joshi GP, McCarroll SM, McSwiney M, O'Rourke P and Hurson BJ: Effects of intraarticular morphine on analgesic requirements after anterior cruciate ligament repair. Reg Anesth. 18:254–257. 1993.PubMed/NCBI
12	Whitford A, Healy M, Joshi GP, McCarroll SM and O'Brien TM: The effect of tourniquet release time on the analgesic efficacy of intraarticular morphine 1after arthroscopic knee surgery. Anesth Analg. 84:791–793. 1997. View Article : Google Scholar : PubMed/NCBI
13	Song HP, Li X, Yu R, Zeng G, Yuan ZY, Wang W, Huang HY and Cai X: Phenotypic characterization of type II collagen-induced arthritis in Wistar rats. Exp Ther Med. 10:1483–1488. 2015. View Article : Google Scholar : PubMed/NCBI
14	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
15	Coggeshall RE, Zhou S and Carlton SM: Opioid receptors on peripheral sensory axons. Brain Res. 764:126–132. 1997. View Article : Google Scholar : PubMed/NCBI
16	Yang M, Xiao C, Wu Q, Niu M, Yao Q, Li K, Chen Y, Shi C, Chen D, Feng G and Xia C: Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-kappa B and p38 MAPK alpha in collagen-induced arthritis in rat. J Ethnopharmacol. 127:264–273. 2010. View Article : Google Scholar : PubMed/NCBI
17	Dayer JM, Beutler B and Cerami A: Cachectin/tumor necrosis factor stimulates collagenase and prostaglandin E2 production by human synovial cells and dermal fibroblasts. J Exp Med. 162:2163–2168. 1985. View Article : Google Scholar : PubMed/NCBI
18	Furst DE, Breedveld FC, Kalden JR, Smolen JS, Burmester GR, Bijlsma JW, Dougados M, Emery P, Keystone EC, Klareskog L and Mease PJ: Updated consensus statement on biological agents, specifically tumour necrosis factor {alpha} (TNF{alpha}) blocking agents and interleukin-1 receptor antagonist (IL-1ra), for the treatment of rheumatic diseases, 2005. Ann Rheum Dis. 64 Suppl 4:iv2–i14. 2005. View Article : Google Scholar : PubMed/NCBI
19	Arend WP, Malyak M, Guthridge CJ and Gabay C: Interleukin-1 receptor antagonist: Role in biology. Annu Rev Immunol. 16:27–55. 1998. View Article : Google Scholar : PubMed/NCBI
20	Kishimoto T: Interleukin-6: From basic science to medicine-40 years in immunology. Annu Rev Immunol. 23:1–21. 2005. View Article : Google Scholar : PubMed/NCBI
21	Bertolini DR, Nedwin GE, Bringman TS, Smith DD and Mundy GR: Stimulation of bone resorption and inhibition of bone formation in vitro by human tumour necrosis factors. Nature. 319:516–518. 1986. View Article : Google Scholar : PubMed/NCBI
22	Brennan FM, Chantry D, Jackson A, Maini R and Feldmann M: Inhibitory effect of TNF alpha antibodies on synovial cell interleukin-1 production in rheumatoid arthritis. Lancet. 2:244–247. 1989. View Article : Google Scholar : PubMed/NCBI