Open Access

Protective effects of honokiol against oxidative stress‑induced apoptotic signaling in mouse podocytes treated with H2O2

  • Authors:
    • Fang Wu
    • Hangping Yao
    • Fenping Zheng
    • Shengjie Tang
    • Xihua Lin
    • Lin Li
    • Jiaqiang Zhou
    • Hong Li
  • View Affiliations

  • Published online on: June 14, 2018     https://doi.org/10.3892/etm.2018.6313
  • Pages: 1278-1284
  • Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Honokiol (HNK), an important bioactive compound purified from Magnolia officinalis Cortex, has been demonstrated to have manifold beneficial anti‑oxidative, anti‑inflammatory, anti‑bacterial and antitumor pharmacological effects. In the present study, the association of HNK in the signaling mechanism associated with hydrogen peroxide (H2O2)‑induced apoptosis of cultured mouse podocytes was investigated. HNK did not cause significant changes in podocyte viability when its concentration remained below 20 µM. MTS assay and flow cytometry confirmed that H2O2 significantly enhanced the rates of apoptosis while produce significant reduction in viability of podocytes. Following 24 h of pre‑treatment with different concentrations of HNK, the viability of adherent podocytes increased and apoptosis significantly decreased in a dose‑dependent manner below 20 µM. Reverse transcription‑polymerase chain reaction and western blot results indicated that HNK significantly decreased the expression of mRNA and cleaved protein of caspase‑3 and caspase‑9 in podocytes pre‑treated with H2O2. Furthermore, phosphorylation of the signaling molecules protein kinase B (Akt) and extracellular signal‑regulated kinase (Erk) 1/2 appeared to increase following HNK treatment. In conclusion, HNK largely eliminated the role of promoting podocyte apoptosis in an oxidative stress environment, which was a protective factor on podocytes cultured with H2O2. The anti‑oxidative stress mechanisms of HNK are partly due to suppressing the expression of caspase‑3 and caspase‑9 and upregulating phosphorylated‑Akt and ‑Erk 1/2.
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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wu F, Yao H, Zheng F, Tang S, Lin X, Li L, Zhou J and Li H: Protective effects of honokiol against oxidative stress‑induced apoptotic signaling in mouse podocytes treated with H2O2. Exp Ther Med 16: 1278-1284, 2018
APA
Wu, F., Yao, H., Zheng, F., Tang, S., Lin, X., Li, L. ... Li, H. (2018). Protective effects of honokiol against oxidative stress‑induced apoptotic signaling in mouse podocytes treated with H2O2. Experimental and Therapeutic Medicine, 16, 1278-1284. https://doi.org/10.3892/etm.2018.6313
MLA
Wu, F., Yao, H., Zheng, F., Tang, S., Lin, X., Li, L., Zhou, J., Li, H."Protective effects of honokiol against oxidative stress‑induced apoptotic signaling in mouse podocytes treated with H2O2". Experimental and Therapeutic Medicine 16.2 (2018): 1278-1284.
Chicago
Wu, F., Yao, H., Zheng, F., Tang, S., Lin, X., Li, L., Zhou, J., Li, H."Protective effects of honokiol against oxidative stress‑induced apoptotic signaling in mouse podocytes treated with H2O2". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1278-1284. https://doi.org/10.3892/etm.2018.6313