SIRT5 regulates pancreatic β‑cell proliferation and insulin secretion in type 2 diabetes

  • Authors:
    • Yongmei Ma
    • Xiaoqiang Fei
  • View Affiliations

  • Published online on: June 13, 2018     https://doi.org/10.3892/etm.2018.6301
  • Pages: 1417-1425
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Abstract

Impaired insulin secretion and insulin resistance are the primary characteristics of type 2 diabetes (T2D). However, the mechanisms underlying insulin secretion failure have yet to be elucidated. The present study demonstrated that sirtuin 5 (SIRT5) is upregulated in patients with T2D and in pancreatic β‑cell lines. It was also revealed that elevated SIRT5 expression is positively associated with age and blood glucose levels, and negatively associated with pancreatic and duodenal homeobox 1 (PDX1) expression. Colony formation and Cell Counting Kit‑8 assays demonstrated that SIRT5 suppressed the proliferation of pancreatic β‑cells in vitro. In addition, downregulation of SIRT5 promoted the secretion of insulin. Additionally, SIRT5 ectopic expression downregulated the expression of PDX1 and the inhibition of SIRT5 upregulated PDX1 expression. Chromatin immunoprecipitation assay analysis demonstrated that SIRT5 may regulate the transcription of PDX1 via H4K16 deacetylation. In conclusion, the results of the present study indicate that SIRT5 may serve an important role in the pathogenesis of T2D, and may present a novel therapeutic target for the treatment of patients with T2D.

Introduction

Diabetes mellitus is a chronic disease that affects ~500 million people worldwide (1). Functional β-cell apoptosis and dysfunctional insulin secretion are the two primary mechanisms that are thought to be involved in the pathogenesis of type 2 diabetes (T2D) (26). Collectively, these mechanisms result in the inability to compensate for insulin resistance. Therefore, enhancing β-cell proliferation and increasing insulin secretion may be novel strategies for the treatment of diabetes.

Sirtuins (SIRTs) are proteins that possess nicotinamide-adenine dinucleotide-dependent deacetylase activity and belong to the histone deacetylase (HDAC) family, which consist of seven different subtypes in mammals (SIRT1-7) (7). SIRTs were originally identified to be involved in lifespan prolongation; however, research has revealed that they serve major roles in a number of additional biological processes, including cell metabolism, aging and tumorigenesis (7).

Although SIRT5 possesses weak deacetylase activity, it catalyzes the modification of acidic lysine residues by glutarylation, succinylation and malonylation (812). Previous studies have identified thousands of potential SIRT5 targets, including metabolic enzymes involved in ketone body synthesis, the tricarboxylic acid cycle, amino acid catabolism, fatty-acid β-oxidation, glycolysis and ATP synthesis (1315). However, the functional role of SIRT5 in T2D remains unknown. SIRT5 regulates the expression of various metabolic enzymes and may also serve a key function in T2D. The present study aimed to determine the roles and underlying mechanism of SIRT5 in T2D.

Materials and methods

Cell culture

MIN6 and INS-1 cells were purchased from the American Type Culture Collection (Manassas, VA, USA) and cultured in low-glucose Dulbecco's modified Eagle's medium (5 mmol/l glucose; HyClone; GE Healthcare Life Sciences, Logan, UT, USA), supplemented with 100 U/ml penicillin, 0.1 mg/ml streptomycin (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 5.5 mM 2-mercaptoethanol and 10% horse serum (Invitrogen; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO2.

Blood sample collection

The present study was approved by the Ethics Committee of Taizhou People's Hospital (Taizhou, China), and written informed consent was provided from patients and healthy volunteers prior to enrolment. Blood samples were collected from 130 patients, including 65 patients with T2D patients (that had not yet received treatment) and 65 healthy volunteers that were admitted to Taizhou People's Hospital between 2015 to 2016, and stored at −80°C prior to use. Healthy control subjects were recruited from age- and gender-matched healthy blood donors. Patients were diagnosed according to the criteria of the American Diabetes Association (16). T2D patients contained 30 males and 36 females, with age range of 11-87 years and an average age of 40±0.55 years. Blood glucose levels were determined using ACCU-CHEK (Roche Diagnostics GmbH, Mannheim, Germany).

Plasmid and transfection

pcDNA3.1-SIRT5 and pcDNA3.1-pancreatic and duodenal homeobox 1 (PDX1) were purchased from Vigene Biosciences (Rockville, MD, USA). The inactive form of the SIRT5 deacetylase (T69A) was mutated using a Fast Mutagenesis System (Beijing Transgen Biotech Co., Ltd., Beijing, China) according to the manufacturer's protocol. RNAi-resistant SIRT5 (SIRT5R) was mutated using Fast Mutagenesis System according to the sequence of SIRT5 siRNA. For overexpressing SIRT5, a total of 2.5 µg vector (pcDNA3.1) and indicated plasmid were transfected into MIN6 and INS-1 cells using lipo 2000 (Thermo Fisher Scientific Inc.). Following transfection for 48 h, cells were collected and used for subsequent experiments.

RNA interference

A total of 4×105 INS-1 or MIN6 cells were transfected with 50 nM SIRT5 small interfering RNA (siSIRT5) or scrambled siRNA (SCR, Sigma-Aldrich, St. Louis, MO, USA) using the DharmaFECT 1 kit (Thermo Fisher Scientific Inc.) according to the manufacturers' protocol. Following transfection for 48 h, the expression of SIRT5 was determined using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blotting. Each experiment was repeated three times. SIRT5 siRNA was synthesized form Sigma-Aldrich (Darmstadt, Germany). The sequences for SIRT5 were as follows: siRNA#1, GAGAAUUACAAGAGUCCAAUU and siRNA#2, GGAGAUCCAUGGUAGCUUAUU.

RT-qPCR

Total RNA from blood samples and INS-1 or MIN6 cells was extracted using the TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. A total of 2 µg RNA was reverse transcribed into cDNA using the EasyScript First-Strand cDNA Synthesis SuperMix kit (TransGen Biotech Co., Ltd., Beijing, China) according to the manufacturer's protocol. A SYBR green Mix (Roche Diagnostics GmbH) was used to perform RT-qPCR assay. The thermal cycling parameters were as follows: 33 cycles of denaturation at 95°C for 4 min, annealing at 55°C for 30 sec followed by extension at 72°C for 8 min. The following primers were used: SIRT5, forward, 5′-AAATAACTAAAGCCCGCCTC-3′, and reverse, 5′-CCACTTTCTGCACTAACACC-3′; PDX1, forward, 5′-CCTTTCCCATGGATGAAGTC-3′, and reverse, 5′-GTGAGATGTACTTGTTGAATAGGA-3′; glyceraldehyde-3-phosphate dehydrogenase (GAPDH), forward, 5′-ATGATGACATCAAGAAGGTGG-3′, and reverse, 5′-TTGTCATACCAGGAAATGAGC-3′. The relative mRNA expression was normalized to that of GAPDH and quantified using the 2−ΔΔCt method (17). Each experiment was repeated three times.

Western blotting

Cells were harvested and lysed in radioimmunoprecipitation assay (RIPA, Beyotime Institute of Biotechnology, Jiangsu, China) buffer, and the protein concentration of sample extracts was determined using a BCA protein assay kit (Pierce; Thermo Fisher Scientific, Inc.). A total of 45 µg protein was loaded per lane and electrophoresed by 8% SDS-PAGE. Samples were then transferred to a polyvinylidene fluoride membrane. Membranes were blocked in 5% non-fat milk at room temperature for 1 h. Membranes were then incubated with primary antibodies against SIRT5 (1:1,000; cat. no. ab108968), PDX1 (1:1,000; cat. no. ab134150) or β-actin (1:7,000; cat. no. ab8226) overnight at 4°C (All Abcam, Cambridge, UK). Following washing three times with tris-buffered saline Tween 20, membranes were incubated with secondary horseradish peroxidase-conjugated antibodies (goat anti mouse, 1:3,000; ab6789; Abcam, Cambridge, UK; goat anti rabbit, 1:5,000, ab205718; Abcam, Cambridge, UK) at room temperature for 1 h. Specific protein bands were visualized using a BeyoECL Plus ECL kit (Beyotime Institute of Biotechnology). Image J software (version 1.48; National Institutes of Health, Besthesda, MD, USA) was used for densitometry.

CCK-8 assay

A CCK-8 assay was used to investigate the effect of SIRT5 expression on cell proliferation. INS-1 and MIN6 cells were transfected with 2.5 µg pcDNA 3.1 vector, SIRT5 plasmid (Vigene Biosciences, Rockville, MD, USA) or SIRT5 and PDX1 plasmid, 50 nM scramble siRNA (SCR) or SIRT5 siRNA (siSIRT5, Sigma-Aldrich, St. Louis, MO, USA), respectively. Following transfection for 48 h, a total of 3×103 cells were seeded into 96-well plates with 200 µl low-glucose Dulbecco's Modified Eagle's medium (5 mmol/l glucose; HyClone; GE Healthcare Life Sciences) supplemented with 10% horse serum (Invitrogen; Thermo Fisher Scientific, Inc.). Subsequently, 20 µl CCK-8 reagent (Beyotime Institute of Biotechnology) was added to each well and cells were incubated for a further 1 h at 37°C. Finally, the absorbance at 450 nm was read using a microplate reader (BioTek Instruments Inc., Winooski, VT, USA). Each experiment was repeated three times.

Colony formation assay

SIRT5 was overexpressed or knocked down in INS-1 and MIN6 cells. Following transfection for 48 h, ~2×104 transfected INS-1 and MIN6 cells were seeded into 6-well plates. Cells were cultured with serum-free low-glucose Dulbecco's modified Eagle's medium for 14 days. Subsequently, cells were fixed with methanol at room temperature for 10 min and stained with 0.5% crystal violet at room temperature for 10 min. The colonies (cells >50) were counted under a light microscope (magnification, 20×). Each experiment was repeated three times. The Image J software (v1.04; National Institutes of Health, Bethesda, MD, USA) was used to assess colony formation

ChIP and qChIP assay

A ChIP assay was performed with 2.5 µg anti-H4K16ac (cat. no. ab109463; Abcam) and goat anti-rabbit IgG (cat. no. ab171870; Abcam) using a ChIP assay kit (Beyotime Institute of Biotechnology) according to the manufacturer's protocol. The antibody-bound DNA was detected in 2% agarose gel. Subsequently, antibody-bound DNA was used to perform qChIP. SYBR green Mix (Roche Diagnostics GmbH) was used to perform qPCR. The thermocycling conditions were as follows: 30 cycles of denaturation at 95°C for 5 min, annealing at 56°C for 30 sec followed by extension at 72°C for 5 min. The following primers were used: PDX1, forward, 5′-AGGGTCTCATTCTGTCGTTC-3′; PDX1, reverse, 5′-GCCTGTAATCCCAGCTACTC-3′. The fold of enrichment was normalized to that of IgG and quantified using the 2−ΔΔCq method (17). Each experiment was repeated three times.

Insulin secretion and insulin content measurements

A total of ~4×105 cells were seeded into a 24-well plate and transfected with a pcDNA 3.1 vector, SIRT5 plasmid or SIRT5 and PDX1 plasmid, scramble siRNA (SCR) or SIRT5 siRNA (siSIRT5). Following transfection for 24 h, MIN6 and INS-1 cells were also incubated with 1 or 20 mM glucose for 90 min and the rate of insulin secretion was detected in each group. Cells were washed with a secretion assay buffer [sub arachnoid block (SAB); 20 mM HEPES (pH 7.2), 2.5 mM CaCl2, 1.16 mM MgSO4, 1.2 mM KH2PO4, 4.7 mM KCl, 25.5 mM NaHCO3, 114 mM NaCl and 0.2% bovine serum albumin (Invitrogen; Thermo Fisher Scientific, Inc.) with 2.8 mM glucose three times. Subsequently, cells were incubated with 1.5 ml SAB at 37°C and 5% CO2 for 2 h. Immediately following incubation, insulin levels were analyzed using the Coat-A-Count insulin RIA kit (Diagnostic Products Corporation, Los Angeles, CA, USA)] according to the manufacturers' protocol. Cells were lysed with RIPA buffer [1% NP-40/Triton X, 0.5% sodium deoxycholate, 0.1% SDS, 150 mM NaCl, 50 mM Tris HCl (pH 8.0), 50 mM NaF and 2 mM EDTA]. The cell lysates were centrifuged at 1×104 g for 5 min at 4°C, and shaken on ice for 30 min. The supernatant was collected and used for the analysis of insulin content. Total protein concentration was measured using a BCA Protein assay kit (Pierce; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The insulin content of each group was normalized to protein concentration. Each experiment was repeated three times.

Incubation of cells with valproic acid (VPA)

INS-1 cells were transfected with vector (pcDNA3.1) or SIRT5 plasmid and cultured with or without 20 mM glucose. A total of 0.1, 0.5 or 1 mM VPA (cat. no. S1168; Selleck Chemicals, Shanghai, China) was added to cells immediately following transfection. The low-glucose Dulbecco's modified Eagle's medium (5 mmol/l glucose) supplemented with 10% horse serum was subsequently removed and VPA-containing medium was refreshed 1 day following transfection. Insulin secretion tests were also performed at 48 h following transfection and treatment with VPA. Each experiment was repeated three times.

Statistical analysis

Each experiment was repeated three times and the results are expressed as the mean ± standard deviation. SPSS 18.0 software (SPSS, Inc., Chicago, IL, USA) was used for statistical analysis. The Student's t-test was used to analyze the differences between two groups and a one-way analysis of variance followed by a Tukey's post hoc test, was used to analyze differences among multiple groups. Associations between SIRT5 expression and blood glucose levels were determined using the Pearson's correlation coefficient. P<0.05 was considered to indicate a statistically significant difference.

Results

Expression of SIRT5 is markedly upregulated in patients with T2D

In order to investigate the role of SIRT5 in diabetes, the expression of SIRT5 in plasma samples obtained from 65 patients with T2D and 65 healthy volunteers was assessed. The result of RT-qPCR analysis revealed that SIRT5 mRNA levels were significantly upregulated in patients with diabetes compared with healthy volunteers (Fig. 1A). It was also determined that the expression of SIRT5 was positively correlated with blood glucose levels (r=0.87; Fig. 1B). In addition, the association between patient clinicopathological features and SIRT5 expression was analyzed, and it was revealed that a high SIRT5 expression was significantly associated with age, but not with gender (Table I).

Table I.

Association between SIRT5 expression and the clinicopathologic features of 65 patients with type 2 diabetes.

Table I.

Association between SIRT5 expression and the clinicopathologic features of 65 patients with type 2 diabetes.

SIRT5 protein expression

CharacteristicNumberLow (n=23)High (n=42)P-value
Age 0.011
  <40261412
  ≥ 039  930
Sex 0.749
  Male301020
  Female351322
Blood glucose level <0.001
  <7.0 mM251510
  >7.0 mM40  832
PDX1 expression <0.001
  Low38  731
  High271611

[i] Data are presented as the mean ± standard deviation. PDX1, pancreatic and duodenal homeobox 1.

SIRT5 suppresses insulin secretion and the proliferation of pancreatic β-cell lines

To elucidate the function of SIRT5 in diabetes, a pcDNA3.1-SIRT5 plasmid was constructed and SIRT5 was overexpressed or knocked down in MIN6 and INS-1 pancreatic β-cell lines. Following a 48 h transfection, the expression of SIRT5 was determined by RT-qPCR and western blotting analyses. SIRT5 was significantly upregulated and downregulated in MIN6 and INS-1 cells transfected with the pcDNA3.1-SIRT5 plasmid and siSIRT5, respectively, when compared with their respective controls (Fig. 2A and B). siSIRT5#2 was demonstrated to be more efficient at repressing SIRT5 than siSIRT5#1, and was therefore utilized in subsequent experiments. A colony formation assay was performed to assess the effect of SIRT5 expression on the proliferation of pancreatic β-cell lines. The results demonstrated that ectopic SIRT5 expression significantly suppressed MIN6 and INS-1 cell colony formation when compared with controls, whereas inhibition of SIRT5 expression had the opposite effect (Fig. 2C). In addition, the results of the CCK-8 assay indicated that upregulation of SIRT5 significantly inhibited MIN6 and INS-1 cell proliferation when compared with controls, whereas SIRT5 inhibition demonstrated the opposite effect (Fig. 2D). It was also demonstrated that overexpression of SIRT5 significantly decreased the level of insulin secretion following exposure to glucose when compared with controls, whereas inhibition of SIRT5 expression significantly increased insulin secretion (Fig. 2E). Therefore, the results demonstrated that SIRT5 downregulation facilitates insulin secretion and the proliferation of pancreatic β-cell lines.

PDX1 is transcriptionally regulated by SIRT5

As a HDAC enzyme, SIRT5 has been demonstrated to regulate the expression of genes (7). The protein PDX1 serves a number of key roles in the pathogenesis of diabetes (18). The authors of the present study therefore hypothesized that SIRT5 may regulate the expression of PDX1 in patients with diabetes. To verify this hypothesis, SIRT5 was overexpressed or knocked down in MIN6 and INS-1 cells and the expression of PDX1 was determined using RT-qPCR and western blotting analyses. The results demonstrated that PDX1 mRNA and protein levels were regulated by SIRT5 (Fig. 3A and B). SIRT5 ectopic expression and inhibition significantly reduced and increased the expression of PDX1, respectively (Fig. 3A and B). To investigate whether the effects of SIRT5 on the expression of PDX1 were dependent on its deacetylase activity, an inactive form of the SIRT5 deacetylase (T69A) was overexpressed. It was demonstrated that ectopic expression of SIRT5 (T69A) had no significant effect on the expression of PDX1 (Fig. 3A and B). Subsequently, a ChIP assay was performed to determine whether SIRT5 regulates the transcription of PDX1 expression through H4K16 deacetylation. The results revealed that occupation of H4K16ac in the PDX1 promoter region was significantly increased following knock down of SIRT5 (Fig. 3C). However, reconstituting the expression of SIRT5R in MIN6 cells could partially offset the effect of SIRT5-depletion (Fig. 3C). Similar results were also observed in INS-1 cells (Fig. 3C). In addition, the expression of PDX1 in plasma samples obtained from 65 patients with diabetes were assessed. The results revealed that SIRT5 expression was negatively associated with PDX1 expression (Table I). Together, these results suggest that the HDAC activity of SIRT5 may regulate the expression of PDX1 via H4K16 deacetylation.

PDX1 is involved in SIRT5-mediated insulin secretion and pancreatic β-cell proliferation

The results presented thus far have demonstrated that SIRT5 may regulate the transcription of PDX1. Therefore, it was hypothesized that PDX1 may serve as a downstream effector of SIRT5. To verify this hypothesis, the pcDNA3.1-PDX1 plasmid was constructed to increase the expression of PDX1 in MIN6 and INS-1 cells. Western blotting and RT-qPCR analyses were employed to analyze PDX1 protein and mRNA levels, respectively. It was demonstrated that the protein and mRNA levels of PDX1 were significantly increased in MIN6 and INS-1 cells transfected with a PDX1 when compared with controls (Fig. 4A and B). The involvement of PDX1 in SIRT5-mediated insulin secretion and pancreatic β-cell proliferation was then determined. It was demonstrated that the simultaneous overexpression of SIRT5 and PDX1 offset the effect of SIRT5 overexpression in INS-1 and MIN6 cells (Fig. 4C). When cells were simultaneously overexpressed with SIRT5 and PDX1, the glucose-stimulated secretion of insulin was increased compared with the cells overexpressing SIRT5 (Fig. 4C). Similarly, the CCK-8 assay results demonstrated that combined SIRT5 and PDX1 overexpression significantly reduced the effect of the SIRT5 overexpression on cell proliferation (Fig. 4D). These results indicate that PDX1 may function as a downstream target of SIRT5 and mediate pancreatic β-cell proliferation and insulin secretion.

VPA increases PDX1 expression and insulin secretion

Due to the HDAC activity of SIRT, the effect of VPA treatment (a HDAC inhibitor) on pancreatic β-cells was assessed. MIN6 and INS-1 cells incubated with 0.1, 0.5 and 1 mM VPA for 48 h exhibited a significant increase in the expression of PDX1 (Fig. 5A). In addition, treatment with increasing concentrations of VPA was associated with a dose-dependent increase in insulin secretion following exposure to glucose and SIRT5 overexpression decreased insulin secretion compared with the control group (Fig. 5B). The results therefore suggest that VPA may be a novel therapy for T2D, which is consistent with the results of previous studies (19,20).

Discussion

The dysfunction of β-cells is a primary cause of human T2D and results from the abnormal expression of specific genes involved in regulating metabolic signaling pathways (21,22). Chronically high carbohydrate diets may also contribute to β-cell dysfunction (21). SIRT5 has been demonstrated to serve key roles in various metabolic processes (10,23,24), including deacetylation and desuccinylation of CPS1 by SIRT5, resulting in its increased enzymatic activity, thereby promoting the urea cycle (25). However, the role of SIRT5 in T2D has not yet been elucidated.

The present study demonstrated that SIRT5 was upregulated in patients with T2D and that the expression of SIRT5 was associated with high blood glucose levels. It was also revealed that the expression of SIRT5 was associated with patient age. This may be due to alterations in the expression levels of specific proteins as the body ages, such as SIRT1 (26), resulting in a high expression of SIRT5. To investigate the effect of SIRT5 in T2D in the present study, two pancreatic β-cell lines (MIN6 and INS-1) were utilized. Functional experiments, including colony formation and CCK-8 assays, were performed. The results suggested that SIRT5 inhibition facilitated pancreatic β-cell proliferation and insulin secretion. A previous study demonstrated that SIRT5 facilitates cancer cell growth and drug resistance in non-small cell lung cancer (27). This contradicts the results of the present study; however, it should be considered that diabetes and cancer are two different diseases. The present study hypothesized that there are also many factors that may contribute to the different functions of SIRT5, including cell microenvironment, metabolism and oncogene activation.

PDX1 is the earliest tissue-selective transcription factor expressed in the developing primordium, and is essential to formation of all pancreatic cell types and the activity of adult islet β cells (28). Thus, mice and humans that completely lack Pdx1 function are apancreatic, while haploinsufficiency primarily affects islet β cells following birth (28). Moreover, β cell-specific inactivation of Pdx1 in the adult mouse causes severe hyperglycemia and loss of cell identity, with these cells transdifferentiating to an islet α-like cell capable of secreting the glucagon hormone (18). Previous studies have demonstrated that PDX1 serves a primary role in diabetes and that the downregulation of PDX1 expression aggravates diabetes mellitus (29,30). The results of the present study demonstrated that SIRT5 may regulate the transcription of PDX1 through its deacetylase activity. Despite SIRT5 only possessing weak deacetylase activity, it was demonstrated that PDX1 is negatively regulated by SIRT5. A previous study suggested that SIRT5 may function through its de-succinylase activity (31). However, the role of succinylation in cancer and metabolic diseases remains unknown. It is therefore important to assess alterations in the level of specific histone or protein succinylation in patients with diabetes mellitus using mass spectrometry in future studies.

Previous studies have demonstrated that the HDAC inhibitor, VPA, increases insulin secretion in vivo (19,20). Consistent with these results, the present study revealed that VPA facilitated the secretion of insulin in two pancreatic β-cell lines. In addition, it was determined that VPA may promote insulin secretion by enhancing the expression of PDX1.

In conclusion, the current study investigated the function of SIRT5 in T2D and the mechanisms underlying it role in insulin secretion and pancreatic β-cell proliferation. The results revealed that SIRT5 may be associated with the pathogenesis of T2D and may serve as a novel biomarker for the diagnosis of T2D.

Acknowledgements

Not applicable.

Funding

No funding was received.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request

Authors' contributions

YM and XF conceived and designed the study, analyzed the data, and wrote the paper. YM constructed the expression plasmids, prepared the proteins, and performed experiments. All authors have read and approved this manuscript.

Ethics approval and consent to participate

The present study was approved by the Ethics Committee of Taizhou People's Hospital (Taizhou, China) and written informed consent was provided from patients and healthy volunteers prior to enrolment.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

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August-2018
Volume 16 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Ma Y and Ma Y: SIRT5 regulates pancreatic β‑cell proliferation and insulin secretion in type 2 diabetes. Exp Ther Med 16: 1417-1425, 2018
APA
Ma, Y., & Ma, Y. (2018). SIRT5 regulates pancreatic β‑cell proliferation and insulin secretion in type 2 diabetes. Experimental and Therapeutic Medicine, 16, 1417-1425. https://doi.org/10.3892/etm.2018.6301
MLA
Ma, Y., Fei, X."SIRT5 regulates pancreatic β‑cell proliferation and insulin secretion in type 2 diabetes". Experimental and Therapeutic Medicine 16.2 (2018): 1417-1425.
Chicago
Ma, Y., Fei, X."SIRT5 regulates pancreatic β‑cell proliferation and insulin secretion in type 2 diabetes". Experimental and Therapeutic Medicine 16, no. 2 (2018): 1417-1425. https://doi.org/10.3892/etm.2018.6301