The function of MMP‑28/TGF‑β induced cell apoptosis in human glioma cells

Wang,Xuepeng; Chen,Xi; Sun,Lin; Bi,Xiaoli; He,Haitao; Chen,Lei; Pang,Jinfeng

doi:10.3892/etm.2018.6566

The function of MMP‑28/TGF‑β induced cell apoptosis in human glioma cells

Authors:
- Xuepeng Wang
- Xi Chen
- Lin Sun
- Xiaoli Bi
- Haitao He
- Lei Chen
- Jinfeng Pang
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Affiliations: Department of Neurosurgery, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China, Department of Neurology, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China, Department of Production, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China, Department of CT, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China, Department of Cycle of Internal, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China
Published online on: August 2, 2018 https://doi.org/10.3892/etm.2018.6566
Pages: 2867-2874
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to assess the expression status of matrix metalloproteinase (MMP)‑28 and to investigate its molecular mechanisms in glioma cells. MicroRNA (miRNA) reverse transcription‑quantitative polymerase chain reaction was used to analyze the expression of MMP‑28 and transforming growth factor (TGF)‑β expression in glioma patients and healthy volunteers. MTT and Transwell assays were conducted to determine cell growth and metastasis, respectively. Annexin V/propidium iodide staining was also employed to measure cell apoptosis. MMP‑28 and TGF‑β protein expression were measured using western Blot analysis. The results indicated that MMP‑28 and TGF‑β expression was downregulated in glioma patients, when compared with the normal group. Overall survival and disease‑free survival of patients with a low expression of MMP‑28 were lower than those with high MMP‑28 expression. Overexpression of MMP‑28 induced TGF‑β protein expression, while downregulation of MMP‑28 suppressed TGF‑β protein expression in glioma cell. The downregulation of MMP‑28 reduced the cell growth and apoptosis of glioma cell via the suppression of TGF‑β. By contrast, upregulation of MMP‑28 induced cell growth and reduced the apoptosis of glioma cells by activating TGF‑β. In addition, the TGF‑β inhibitor attenuated the effects of MMP‑28 in glioma cells. Collectively, the results indicated that MMP‑28 was able to induce TGF‑β in human glioma cells.

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