miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells

Yan,Liang; Ma,Jinzhu; Wang,Yi; Zan,Jiawei; Wang,Zhen; Zhu,Yu; Zhu,Yiping; Ling,Liefeng; Cao,Long; Liu,Xin; Li,Shu; Xu,Lei; Qi,Zhilin; Nie,Liuwang; Zhang,Yao

doi:10.3892/etm.2018.6752

miR‑21‑5p induces cell proliferation by targeting TGFBI in non‑small cell lung cancer cells

Authors:
- Liang Yan
- Jinzhu Ma
- Yi Wang
- Jiawei Zan
- Zhen Wang
- Yu Zhu
- Yiping Zhu
- Liefeng Ling
- Long Cao
- Xin Liu
- Shu Li
- Lei Xu
- Zhilin Qi
- Liuwang Nie
- Yao Zhang
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Affiliations: Department of Biopharmaceuticals, College of Life Sciences, Anhui Normal University, Wuhu, Anhui 241002, P.R. China, Department of Biochemistry, Provincial Key Laboratory of Biological Macro‑molecules Research, Wannan Medical College, Wuhu, Anhui 241002, P.R. China, Department of Clinical Teaching, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, P.R. China, Department of Chemistry, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241002, P.R. China
Published online on: September 18, 2018 https://doi.org/10.3892/etm.2018.6752
Pages: 4655-4663
Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The mortality rate of non‑small cell lung cancer (NSCLC) remains high worldwide. miR‑21‑5p plays an important part in many cancer types, including NSCLC. However, the effect of miR‑21‑5p in NSCLC tumorigenesis remains poorly understood. The present study investigated whether miR‑21‑5p promoted NSCLC cell proliferation in vitro. In order to study the molecular mechanism by which miR‑21‑5p contributes to NSCLC progression, three bioinformatics algorithms were used to predict the genes which miR‑21‑5p targeted. TGFBI was identfieid as a putative direct target in NSCLC cells via the luciferase reporter assay. Furthermore, miR‑21‑5p downregulated TGFBI protein expression by a post‑transcriptional mechanism via western blotting and a reverse transcription‑quantitative polymerase chain reaction analysis. Finally, TGFBI exhibited opposing effects to those of miR‑21‑5p on NSCLC cells, suggesting that miR‑21‑5p may promote cell proliferation by negative regulation of TGFBI. These results suggest miR‑21‑5p promote the proliferation of NSCLC cells via inhibiting TGFBI expression.

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