Expression of PTEN‑long nephritis and its effect on renal inflammation

  • Authors:
    • Hui Wang
    • Qingxia Yu
    • Lin Wang
    • Yongwei Li
    • Mao Xie
    • Youyi Lu
    • Yupeng Cui
  • View Affiliations

  • Published online on: December 5, 2018     https://doi.org/10.3892/etm.2018.7049
  • Pages: 1405-1411
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Abstract

Based on the important functions of phosphatase and tensin homolog (PTEN)‑Long for renal diseases, the present study aimed to investigate the expression of PTEN‑Long in patients and mice with nephritis and its effect on nephritis. Expression levels of PTEN‑Long in serum of patients with nephritis, renal cell carcinoma (RCC) as well as normal controls, and in serum and renal tissues of mice were measured by western blotting. PTEN‑Long knock‑in and knock‑out mice were constructed via the CRISPR‑Cas9 technique. Intraperitoneal injection of lipopolysaccharide+renal homogenate was performed to construct a mouse nephritis model. Mice were divided into control group, model group, knock‑in group and knock‑out group. A Bio‑Plex system was used to detect secretion of serum inflammatory factors. Expression of inflammatory factors in renal tissues of different groups was detected by reverse transcription semi‑quantitative polymerase chain reaction. Hematoxylin and eosin staining was used to observe the pathological changes of renal tissue. PTEN‑Long was downregulated in patients with nephritis and RCC compared with controls, whereas the expression levels of inflammatory factors were increased. PTEN‑long knock‑in significantly reduced the serum content and expression levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑1β and IL‑18. PTEN‑long knock‑out also decreased the expression levels of TNF‑α and IL‑6 but exhibited no effects on expression of IL‑1β and IL‑18. Compared with knock‑out and model groups, renal tissue inflammation was significantly reduced in knock‑in group. The protein level of PTEN‑Long was significantly lower in serum than in renal tissue. These findings suggest that PTEN‑long can inhibit the progression of nephritis by interacting with inflammatory factors to protect kidney.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Wang H, Yu Q, Wang L, Li Y, Xie M, Lu Y and Cui Y: Expression of PTEN‑long nephritis and its effect on renal inflammation. Exp Ther Med 17: 1405-1411, 2019
APA
Wang, H., Yu, Q., Wang, L., Li, Y., Xie, M., Lu, Y., & Cui, Y. (2019). Expression of PTEN‑long nephritis and its effect on renal inflammation. Experimental and Therapeutic Medicine, 17, 1405-1411. https://doi.org/10.3892/etm.2018.7049
MLA
Wang, H., Yu, Q., Wang, L., Li, Y., Xie, M., Lu, Y., Cui, Y."Expression of PTEN‑long nephritis and its effect on renal inflammation". Experimental and Therapeutic Medicine 17.2 (2019): 1405-1411.
Chicago
Wang, H., Yu, Q., Wang, L., Li, Y., Xie, M., Lu, Y., Cui, Y."Expression of PTEN‑long nephritis and its effect on renal inflammation". Experimental and Therapeutic Medicine 17, no. 2 (2019): 1405-1411. https://doi.org/10.3892/etm.2018.7049