PM2.5 induces cell cycle arrest through regulating mTOR/P70S6K1 signaling pathway

Zhang,Yu; Yang,Dan; Yang,Biao; Li,Bingyu; Guo,Jie; Xiao,Chunling

doi:10.3892/etm.2019.7466

PM2.5 induces cell cycle arrest through regulating mTOR/P70S6K1 signaling pathway

Authors:
- Yu Zhang
- Dan Yang
- Biao Yang
- Bingyu Li
- Jie Guo
- Chunling Xiao
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Affiliations: Key Lab of Environmental Pollution and Microecology of Liaoning Province, Shenyang Medical College, Shenyang, Liaoning 110034, P.R. China
Published online on: April 3, 2019 https://doi.org/10.3892/etm.2019.7466
Pages: 4371-4378
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Fine particulate matter (PM2.5) pollution has become a serious problem in China. This study aims to elucidate the toxicity mechanism of PM2.5. Protein levels were detected by western blotting and RT‑qPCR, and cell cycle was detected by flow cytometry. The results showed that exposure to PM2.5 induces cell cycle arrest and downregulation of the expression of cyclin D1 protein. Moreover, the protein expression of thymidylate synthase (TS) enzyme was found to be downregulated and the mRNA expression of TS was upregulated after PM2.5 exposure. Knockout of TS gene promoted cell cycle arrest and downregulation of the expression of cyclin D1 protein after PM2.5 exposure. Our data further revealed that PM2.5 exposure downregulates the expression of TS and cyclin D1 partially through the downregulation of the mammalian target of rapamycin (mTOR)/P70S6K1 signaling pathway. Thus, these findings indicate that PM2.5‑induced cell cycle arrest might be due to the downregulation of mTOR/P70S6K1 signaling pathway, and thus inhibits the expression of TS protein.

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