Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro

  • Authors:
    • Xiao-Hui Deng
    • Hai-Yan Song
    • Ying-Feng Zhou
    • Guo-Yan Yuan
    • Feng-Jin Zheng
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  • Published online on: September 3, 2013     https://doi.org/10.3892/etm.2013.1285
  • Pages: 1155-1158
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Abstract

Quercetin is a hydrophobic agent with potential anticancer activity. The aim of the present study was to observe the effects of quercetin on the proliferation of the breast cancer cell line MCF-7 and the gene expression of survivin. The molecular mechanism underlying the antiproliferative effect of quercetin was also investigated. MCF-7 breast cancer cells were treated with various concentrations of quercetin. The inhibitory effect of quercetin on proliferation was detected using the 3-(4,5-dimethylthiazol-2-yl)‑2,5-diphenyltetrazolium bromide (MTT) method and the inhibition rate was calculated. Cellular apoptosis was detected by immunocytochemistry and survivin mRNA expression levels were observed using reverse transcription‑polymerase chain reaction (RT-PCR). Western blot analysis was used to analyze changes in the expression levels of survivin protein. Quercetin induced the apoptosis of MCF-7 cells and inhibited the proliferation of the MCF-7 breast cancer cells in a time- and concentration-dependent manner. The mRNA and protein expression levels of survivin were reduced as the concentration of quercetin increased. Quercetin inhibited the growth of MCF-7 cells and promoted apoptosis by inducing G0/G1 phase arrest. It also regulated the expression of survivin mRNA in MCF-7 cells, which may be the mechanism underlying its antitumor effect.
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November 2013
Volume 6 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Deng X, Song H, Zhou Y, Yuan G and Zheng F: Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Exp Ther Med 6: 1155-1158, 2013
APA
Deng, X., Song, H., Zhou, Y., Yuan, G., & Zheng, F. (2013). Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro. Experimental and Therapeutic Medicine, 6, 1155-1158. https://doi.org/10.3892/etm.2013.1285
MLA
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6.5 (2013): 1155-1158.
Chicago
Deng, X., Song, H., Zhou, Y., Yuan, G., Zheng, F."Effects of quercetin on the proliferation of breast cancer cells and expression of survivin in vitro". Experimental and Therapeutic Medicine 6, no. 5 (2013): 1155-1158. https://doi.org/10.3892/etm.2013.1285