Pien Tze Huang inhibits hypoxia‑induced epithelial‑mesenchymal transition in human colon carcinoma cells through suppression of the HIF‑1 pathway

Corrigendum in: /10.3892/etm.2024.12458

  • Authors:
    • Hongwei Chen
    • Aling Shen
    • Yuchen Zhang
    • Youqin Chen
    • Jiumao Lin
    • Wei Lin
    • Thomas Sferra
    • Jun Peng
  • View Affiliations

  • Published online on: February 17, 2014     https://doi.org/10.3892/etm.2014.1549
  • Pages: 1237-1242
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Abstract

Hypoxia‑induced activation of the hypoxia‑inducible factor 1 (HIF‑1) signaling pathway is frequently observed in solid tumors and is strongly associated with numerous pathophysiological processes, including the induction of epithelial‑mesenchymal transition (EMT), which result in cancer progression and metastasis. Thus, inhibiting EMT through the suppression of the HIF‑1 pathway may be a promising strategy for anticancer chemotherapy. Pien Tze Huang (PZH), a well‑established traditional Chinese medicine has been prescribed for >450 years and has been used for centuries to clinically treat various types of human cancer. We previously reported that PZH suppresses multiple intracellular signaling pathways and thereby promotes the apoptosis of cancer cells and the inhibition of cell proliferation and tumor angiogenesis. In the present study, to further explore the mechanisms underlying the antitumor action of PZH, HCT‑8 human colon carcinoma cells were cultured under hypoxic conditions and the effect of PZH on hypoxia‑induced EMT was assessed. Hypoxia was found to induce EMT‑associated morphological changes in HCT‑8 cells, including loss of cell adhesion and the development of spindle‑shaped fibroblastoid‑like morphology. In addition, hypoxia was observed to reduce the expression of the epithelial marker E‑cadherin, but increase that of the mesenchymal marker N‑cadherin. In addition, hypoxia significantly enhanced HCT‑8 cell migration and invasion and induced the activation of the HIF‑1 pathway. However, treatment of the HCT‑8 cells with PZH significantly inhibited the hypoxia‑mediated EMT and HIF‑1 signaling. These findings suggest that PZH inhibits hypoxia‑induced cancer EMT through the suppression of the HIF‑1 pathway, which may be one of the molecular mechanisms by which PZH exerts its antitumor activity.
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May-2014
Volume 7 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen H, Shen A, Zhang Y, Chen Y, Lin J, Lin W, Sferra T and Peng J: Pien Tze Huang inhibits hypoxia‑induced epithelial‑mesenchymal transition in human colon carcinoma cells through suppression of the HIF‑1 pathway Corrigendum in /10.3892/etm.2024.12458. Exp Ther Med 7: 1237-1242, 2014.
APA
Chen, H., Shen, A., Zhang, Y., Chen, Y., Lin, J., Lin, W. ... Peng, J. (2014). Pien Tze Huang inhibits hypoxia‑induced epithelial‑mesenchymal transition in human colon carcinoma cells through suppression of the HIF‑1 pathway Corrigendum in /10.3892/etm.2024.12458. Experimental and Therapeutic Medicine, 7, 1237-1242. https://doi.org/10.3892/etm.2014.1549
MLA
Chen, H., Shen, A., Zhang, Y., Chen, Y., Lin, J., Lin, W., Sferra, T., Peng, J."Pien Tze Huang inhibits hypoxia‑induced epithelial‑mesenchymal transition in human colon carcinoma cells through suppression of the HIF‑1 pathway Corrigendum in /10.3892/etm.2024.12458". Experimental and Therapeutic Medicine 7.5 (2014): 1237-1242.
Chicago
Chen, H., Shen, A., Zhang, Y., Chen, Y., Lin, J., Lin, W., Sferra, T., Peng, J."Pien Tze Huang inhibits hypoxia‑induced epithelial‑mesenchymal transition in human colon carcinoma cells through suppression of the HIF‑1 pathway Corrigendum in /10.3892/etm.2024.12458". Experimental and Therapeutic Medicine 7, no. 5 (2014): 1237-1242. https://doi.org/10.3892/etm.2014.1549