Osteopontin inhibits HIF-2α mRNA expression in osteoarthritic chondrocytes

  • Authors:
    • Chao Cheng
    • Fang‑Jie Zhang
    • Jian Tian
    • Min Tu
    • Yi‑Lin Xiong
    • Wei Luo
    • Yu‑Sheng Li
    • Bing‑Bing Song
    • Shu‑Guang Gao
    • Guang‑Hua Lei
  • View Affiliations

  • Published online on: April 20, 2015     https://doi.org/10.3892/etm.2015.2434
  • Pages: 2415-2419
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Abstract

The aim of the present study was to investigate the in vitro effect of osteopontin (OPN) on the expression of hypoxia‑inducible factor‑2α (HIF‑2α) in chondrocytes and the role of OPN in osteoarthritis (OA). Cartilage was purified from the tibial surfaces of patients with OA of the knee and cultured in vitro to obtain chondrocytes. Recombinant human OPN (rhOPN) and OPN small interfering RNA (siRNA) were used to treat the chondrocytes, and the changes in the expression levels of the HIF‑2α gene were measured. An anti‑CD44 blocking monoclonal antibody (mAb) was used to determine the probable ligand‑receptor interactions. Reverse transcription‑quantitative polymerase chain reaction assays were designed and validated with SYBR® Green dyes for the simultaneous quantification of the mRNA expression levels of OPN and HIF‑2α. The mRNA expression level of HIF‑2α was markedly decreased in the rhOPN‑treated group compared with that in the control group; by contrast, OPN siRNA increased HIF‑2α gene expression. CD44 blocking mAb suppressed the inhibitory effect of OPN on HIF‑2α mRNA expression. The results of the present study suggest that OPN may play a protective role in OA by inhibiting HIF‑2α gene expression in osteoarthritic chondrocytes through CD44 interaction.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Cheng C, Zhang FJ, Tian J, Tu M, Xiong YL, Luo W, Li YS, Song BB, Gao SG, Lei GH, Lei GH, et al: Osteopontin inhibits HIF-2α mRNA expression in osteoarthritic chondrocytes. Exp Ther Med 9: 2415-2419, 2015.
APA
Cheng, C., Zhang, F., Tian, J., Tu, M., Xiong, Y., Luo, W. ... Lei, G. (2015). Osteopontin inhibits HIF-2α mRNA expression in osteoarthritic chondrocytes. Experimental and Therapeutic Medicine, 9, 2415-2419. https://doi.org/10.3892/etm.2015.2434
MLA
Cheng, C., Zhang, F., Tian, J., Tu, M., Xiong, Y., Luo, W., Li, Y., Song, B., Gao, S., Lei, G."Osteopontin inhibits HIF-2α mRNA expression in osteoarthritic chondrocytes". Experimental and Therapeutic Medicine 9.6 (2015): 2415-2419.
Chicago
Cheng, C., Zhang, F., Tian, J., Tu, M., Xiong, Y., Luo, W., Li, Y., Song, B., Gao, S., Lei, G."Osteopontin inhibits HIF-2α mRNA expression in osteoarthritic chondrocytes". Experimental and Therapeutic Medicine 9, no. 6 (2015): 2415-2419. https://doi.org/10.3892/etm.2015.2434