Alteration of α-amylase secretion from rat parotid cells in the absence of extracellular bicarbonate
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- Published online on: August 1, 2003 https://doi.org/10.3892/ijmm.12.2.199
- Pages: 199-200
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Abstract
A sufficient availability of the bicarbonate anion is required to allow a normal process of glucose-stimulated insulin release. Thus, both the removal of extracellular bicarbonate and the inhibition of carbonic anhydrase by acetazolamide suppress the insulinotropic action of glucose. In the present study, a comparable situation is documented in rat parotid cells. Thus, the absence of extracellular bicarbonate is shown to decrease by about 50% basal α-amylase secretion, as well as carbamylcholine- and isoproterenol-stimulated α-amylase output, without suppressing the enhancing action of the two agents. It is proposed, therefore, that the HCO3−anion participates in the secretory sequence at sites distal to the identification of secretagogues in both endocrine and exocrine cells.