The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization

  • Authors:
    • Min Young Seo
    • Sun Young Rha
    • Sang Hwa Yang
    • Sang Cheol Kim
    • Gui Youn Lee
    • Chan Hee Park
    • Woo Ick Yang
    • Joong Bae Ahn
    • Byung Woo Park
    • Hyun Cheol Chung
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/ijmm.13.1.17
  • Pages: 17-24
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Abstract

The nature of genetic alterations in bilateral breast cancer (BBC) associated with the distinctive development of a second primary tumor or a metastatic lesion is not clearly established. In this study, patterns of promoter methylation and gene copy number changes were assessed for their utility in the distinction of two types of BBC (synchronous and metachronous). Seven cases of synchronous and five cases of metachronous breast cancer tissues were used in X chromosome inactivation assay to assess the methylation pattern of human androgen receptor gene. X chromosome inactivation assay alone did not provide enough information to distinguish the genetic origins of synchronous and metachronous BBC. When four pairs of paraffin-embedded BBC tissues were used in cDNA array-based CGH with placenta DNA as a reference, higher DNA copy number changes were observed from metachronous pairs (9.0%) than from synchronous pairs (3.1%). From the two cases of metachronous pairs tested, 44 genes were found to be commonly modulated in gene copy numbers in a cancer detected later.

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January 2004
Volume 13 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Seo, M.Y., Rha, S.Y., Yang, S.H., Kim, S.C., Lee, G.Y., Park, C.H. ... Chung, H.C. (2004). The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization. International Journal of Molecular Medicine, 13, 17-24. https://doi.org/10.3892/ijmm.13.1.17
MLA
Seo, M. Y., Rha, S. Y., Yang, S. H., Kim, S. C., Lee, G. Y., Park, C. H., Yang, W. I., Ahn, J. B., Park, B. W., Chung, H. C."The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization". International Journal of Molecular Medicine 13.1 (2004): 17-24.
Chicago
Seo, M. Y., Rha, S. Y., Yang, S. H., Kim, S. C., Lee, G. Y., Park, C. H., Yang, W. I., Ahn, J. B., Park, B. W., Chung, H. C."The pattern of gene copy number changes in bilateral breast cancer surveyed by cDNA microarray-based comparative genomic hybridization". International Journal of Molecular Medicine 13, no. 1 (2004): 17-24. https://doi.org/10.3892/ijmm.13.1.17