Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats

  • Authors:
    • Willy J. Malaisse
    • Laurence Ladriere
    • Ingrid Verbruggen
    • Rudolph Willem
  • View Affiliations

  • Published online on: May 1, 2004     https://doi.org/10.3892/ijmm.13.5.697
  • Pages: 697-703
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Abstract

This study aims at assessing the conversion of exogenous D-__AMB__lsqb;1-13C__AMB__rsqb;fructose, D-__AMB__lsqb;2-13C__AMB__rsqb;fructose or D-__AMB__lsqb;6-13C__AMB__rsqb;-fructose (10 mM) to 13C-enriched and either hydrogenated or deuterated D-glucose, L-lactate and L-alanine released by rat liver cells prepared from Goto-Kakizaki rats and incubated for 120 min in the presence of unlabelled D-glucose (also 10 mM) and D2O. The results of this study are relevant to the relative contribution of fructokinase and hexokinase isoenzyme to the phosphorylation of D-fructose, the capacity of D-glucose to confer to glucokinase positive cooperativity towards D-fructose, the circulation of D-fructose 6-phosphate in the pentose phosphate pathway, the regulation of the cytosolic NADD/NADH ratio, the respective fate of D-fructose-derived D-glyceraldehyde and dihydroxyacetone phosphate, the deuteration of fructose-derived glycolytic intermediates at the phosphoglucoisomerase, phosphomannoisomerase, enolase, pyruvate kinase and glutamate-alanine transaminase levels, and the unequal generation of L-__AMB__lsqb;1-13C__AMB__rsqb;lactate by cells exposed to D-__AMB__lsqb;1-13C__AMB__rsqb;fructose or D-__AMB__lsqb;6-13C__AMB__rsqb;fructose versus D-__AMB__lsqb;2-13C__AMB__rsqb;-fructose.

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May 2004
Volume 13 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Malaisse WJ, Ladriere L, Verbruggen I and Willem R: Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats. Int J Mol Med 13: 697-703, 2004
APA
Malaisse, W.J., Ladriere, L., Verbruggen, I., & Willem, R. (2004). Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats. International Journal of Molecular Medicine, 13, 697-703. https://doi.org/10.3892/ijmm.13.5.697
MLA
Malaisse, W. J., Ladriere, L., Verbruggen, I., Willem, R."Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats". International Journal of Molecular Medicine 13.5 (2004): 697-703.
Chicago
Malaisse, W. J., Ladriere, L., Verbruggen, I., Willem, R."Metabolism of 13C-enriched D-fructose in hepatocytes from Goto-Kakizaki rats". International Journal of Molecular Medicine 13, no. 5 (2004): 697-703. https://doi.org/10.3892/ijmm.13.5.697