Influence of DMBA-induced mammary cancer on the liver CPT I, mit HMG-CoA synthase and PPARα mRNA expression in rats fed a low or high corn oil diet

  • Authors:
    • Raquel Moral
    • Montserrat Solanas
    • Eva Mónica Manzanares
    • Diego Haro
    • Eduard Escrich
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.283
  • Pages: 283-287
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Abstract

Hepatic mitochondrial outer membrane carnitine palmitoyltransferase I (CPT I) and mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (HMG-CoA synthase) enzymes play a key role in regulation of fatty acid oxidation and in ketogenic pathways, respectively. Their expression are regulated by fatty acids mainly by the peroxisome proliferator-activated receptor α (PPARα). To investigate possible mechanisms through which cancer alters the lipid metabolism, we analyzed by Northern blot, the mRNA relative abundance of these proteins in liver from healthy and DMBA-induced mammary tumor-bearing rats fed a low or high corn oil diet. Serum levels of lipids, body weight and mass were also determined. Whereas mRNA steady-state levels of CPT I and mit HMG-CoA synthase were unaffected by the presence of the extra-hepatic tumor, the cancer state seemed to modify the regulation of the expression of these genes by high fat diet. We hypothesize that putative changes in PPARα mRNA levels could have contributed to such alterations. These results, together with changes in serum lipid profiles, body weight and mass, indicate fat mobilization and non-enhanced oxidation rates despite a high-fat feeding. This effect of the cancer state could be related to tumor aggressiveness and suggest a preferential redirection of long-chain fatty acids into energetic and specific pathways of the cancer cells.

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August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Moral R, Solanas M, Manzanares EM, Haro D and Escrich E: Influence of DMBA-induced mammary cancer on the liver CPT I, mit HMG-CoA synthase and PPARα mRNA expression in rats fed a low or high corn oil diet. Int J Mol Med 14: 283-287, 2004
APA
Moral, R., Solanas, M., Manzanares, E.M., Haro, D., & Escrich, E. (2004). Influence of DMBA-induced mammary cancer on the liver CPT I, mit HMG-CoA synthase and PPARα mRNA expression in rats fed a low or high corn oil diet. International Journal of Molecular Medicine, 14, 283-287. https://doi.org/10.3892/ijmm.14.2.283
MLA
Moral, R., Solanas, M., Manzanares, E. M., Haro, D., Escrich, E."Influence of DMBA-induced mammary cancer on the liver CPT I, mit HMG-CoA synthase and PPARα mRNA expression in rats fed a low or high corn oil diet". International Journal of Molecular Medicine 14.2 (2004): 283-287.
Chicago
Moral, R., Solanas, M., Manzanares, E. M., Haro, D., Escrich, E."Influence of DMBA-induced mammary cancer on the liver CPT I, mit HMG-CoA synthase and PPARα mRNA expression in rats fed a low or high corn oil diet". International Journal of Molecular Medicine 14, no. 2 (2004): 283-287. https://doi.org/10.3892/ijmm.14.2.283