Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH

  • Authors:
    • Michèle Cottier
    • Andrei Tchirkov
    • Bernard Perissel
    • Michel Giollant
    • Lydia Campos
    • Philippe Vago
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijmm.14.4.483
  • Pages: 483-495
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Leukemia cell lines K562, KG1a, U937, HL60, Jurkat and solid tumor cell lines A549 and M4Beu are widely used in studies of cell cycle, apoptosis and adhesion mechanisms in cancer cells. Although the K562 and U937 cell lines were previously subjected to a detailed cytogenetic characterization, only a few molecular cytogenetic investigations have been performed on the other five cell lines. We combined several molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH), multicolor FISH (M-FISH), and comparative genomic hybridization (CGH) to demonstrate the precise genetic aberrations in tumor genomes of these seven cell lines. This information may be useful for multiple studies on these cell lines, providing a genetic basis for the interpretations of experimental findings.

Related Articles

Journal Cover

October 2004
Volume 14 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cottier M, Tchirkov A, Perissel B, Giollant M, Campos L and Vago P: Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH. Int J Mol Med 14: 483-495, 2004.
APA
Cottier, M., Tchirkov, A., Perissel, B., Giollant, M., Campos, L., & Vago, P. (2004). Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH. International Journal of Molecular Medicine, 14, 483-495. https://doi.org/10.3892/ijmm.14.4.483
MLA
Cottier, M., Tchirkov, A., Perissel, B., Giollant, M., Campos, L., Vago, P."Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH". International Journal of Molecular Medicine 14.4 (2004): 483-495.
Chicago
Cottier, M., Tchirkov, A., Perissel, B., Giollant, M., Campos, L., Vago, P."Cytogenetic characterization of seven human cancer cell lines by combining G- and R-banding, M-FISH, CGH and chromosome- and locus-specific FISH". International Journal of Molecular Medicine 14, no. 4 (2004): 483-495. https://doi.org/10.3892/ijmm.14.4.483